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  • Building a cassette-based system for combined eukaryotic gene expression

    Creator:
    Madhira, Raviteja
    Abstract:
    Gene regulatory networks control the state of a cell. Models of such networks have been produced for sea urchin development and suggest that cell type may be defined by distinct network kernels. Such kernels are composed of a set of sequence specific DNA-binding transcription factors (SSTFs) that maintain each other’s...
    Resource Type:
    Honors College Thesis
    Full Text:
    presented on August 26, 2009. Title: Building a cassette-based system for combined eukaryotic gene

  • Metagenomics, barcoding, and metabarcoding statistical analysis to facilitate integrative genomic studies of microbial and eukaryotic assemblages

    Creator:
    Senn, Savanah
    Abstract:
    The body of work aims to contribute to the area of Quantitative analysis in Environmental Sciences, and is focused on the microbiome. The underlying theme for the dissertation is methods and workflow. The unique bioinformatics workflow used for the analysis of genomic data was used to answer distinct and pressing...
    Resource Type:
    Dissertation
    Full Text:
    Genomic Studies of Microbial and Eukaryotic Assemblages Abstract approved

  • Investigating Protein-lipid-membrane Interactions in Plant Cells using Bimolecular Fluorescence Complementation

    Creator:
    Tao, Kai
    Abstract:
    The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...
    Resource Type:
    Dissertation
    Full Text:
    -cell imaging by confocal microscopy and image analysis .................................. 41

  • A multiscale approach to bacterial adhesion

    Creator:
    Ostvar, Sassan
    Abstract:
    This work is an attempt to develop a simple physical description for the initial interactions between a bacterial cell and a substrate surface. This is done by adopting a view that regards bacterial surface biomolecules not as secondary and add-on contributors to the potential energy function that characterizes the overall...
    Resource Type:
    Masters Thesis
    Full Text:
    physical description for the initial interactions between a bacterial cell and a substrate surface. This

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation
    Full Text:
    degree of Doctor of Philosophy in Microbiology presented on May 27 th , 2016. Title: Single-Cell

  • Gene Networks of the Skeletal Muscle Cell

    Creator:
    Singh, Arun J.
    Abstract:
    Skeletal muscle is the largest organ in the body by mass, comprising roughly 40% of total bodyweight in adults. It plays diverse and unique roles that include movement, locomotion, and support for posture and internal organs, among others. The structural foundation for all skeletal muscle in adults is formed early...
    Resource Type:
    Dissertation
    Full Text:
    . Title: Gene Networks of the Skeletal Muscle Cell Abstract approved

  • Bacterial pathogens and associated toxins involved in erythrophore cell aggregation

    Creator:
    Hutchison, Janine R.
    Abstract:
    Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as...
    Resource Type:
    Dissertation
    Full Text:
    Erythrophore Cell Aggregation Chapter 1 Introduction Cell-based biosensors utilize eukaryotic cells to

  • Cell cycle regulation of DNA precursor accumulation in the yeast Saccharomyces cerevisiae

    Creator:
    Koc, Ahmet
    Abstract:
    In budding yeast, many of the genes that encode enzymes required for DNA precursor synthesis (MCB genes) are expressed under cell cycle control in late G1/S. The relationship between MCB gene expression, dNTP synthesis and DNA synthesis was investigated by using α factor-synchronized Saccharomyces cerevisiae. The levels of all four...
    Resource Type:
    Dissertation
    Full Text:
    polymerization. 8 CELL CYCLE CHECKPOINTS Eukaryotic cells respond to DNA damage and replication blocks by

  • An emerging role for the exocyst : plant morphogenesis

    Creator:
    Cole, Rex A.
    Abstract:
    Plants develop a vast array of cell shapes and sizes by selectively modifying their surrounding cell walls to expand in some regions and not in others. This process of morphogenesis requires the delivery of secretory vesicles to specific locations at the cell periphery, where exocytosis adds new membrane and proteins...
    Resource Type:
    Dissertation
    Full Text:
    Plants develop a vast array of cell shapes and sizes by selectively modifying their surrounding cell

  • The aging endoplasmic reticulum

    Creator:
    Dixon, Brian M.
    Abstract:
    Hallmarks of aging include the accumulation of aberrant proteins and a lower resistance to stresses. Because the endoplasmic reticulum (ER) functions to fold proteins and possesses unique stress sensing pathways, the central hypothesis of this dissertation is that the ER is a target of cellular aging and significantly underlies these...
    Resource Type:
    Dissertation
    Full Text:
    hepatocyte cell culture model that maintains the aging phenotype. The ER luminal oxidation/reduction