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Accelerated Identification of Proteins by Mass Spectrometry by Employing Covalent Pre-Gel Staining with Uniblue A

Creator:

Mata-Gomez, Marco A., Yasui, Matthew T., Guerrero-Rangel, Armando, Valdes-Rodrıguez, Silvia, and Winkler, Robert

Abstract:

Background: The identification of proteins by mass spectrometry is a standard method in biopharmaceutical quality control and biochemical research. Prior to identification by mass spectrometry, proteins are usually pre-separated by electrophoresis. However, current protein staining and de-staining protocols are tedious and time consuming, and therefore prolong the sample preparation time...

Resource Type:

Article

Full Text:

protein in the gel was fixed in 40% (v/v) ethanol and 10% (v/v) acetic acid for 20 min and stained with
Selective Ablation of Ctip2/Bcl11b in Epidermal Keratinocytes Triggers Atopic Dermatitis-Like Skin Inflammatory Responses in Adult Mice

Creator:

Wang, Zhixing, Zhang, Ling-juan, Guha, Gunjan, Li, Shan, Kyrylkova, Kateryna, Kioussi, Chrissa, Leid, Mark, Ganguli-Indra, Gitali, and Indra, Arup K.

Abstract:

Background: Ctip2 is crucial for epidermal homeostasis and protective barrier formation in developing mouse embryos. Selective ablation of Ctip2 in epidermis leads to increased transepidermal water loss (TEWL), impaired epidermal proliferation, terminal differentiation, as well as altered lipid composition during development. However, little is known about the role of Ctip2...

Resource Type:

Article