Water quality impacts both human and ecosystem health. Many pharmaceuticals and personal care products are considered contaminants of emerging concern (CEC) due to their bioactive properties. Among these, metformin—the most commonly prescribed drug for treatment of type 2 diabetes—has been reported at high concentrations (μg L⁻¹) in stream, lake, and...
Many pharmaceuticals have negative effects on biota when released into the environment. For example, recent work has shown that the commonly prescribed antidiabetic drug, metformin (N,N-dimethylbiguanide), has endocrine disrupting effects on fish. However, effects of metformin on aquatic primary producers are poorly known. We exposed cultured isolates of a freshwater...
Biomass gasification is a promising way to obtain green energy, but the gas composition makes it unsuitable for use in traditional technologies (i.e., IC engine). Gas purification over nickel and/or iron catalysts is an attractive alternative. Cellulose-based carbon aerogels (CAGs) have shown suitable physical chemical properties for use as catalyst...
Livestock grazing is the prominent land use in Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis [Beetle & A. Young] S.L. Welsh) steppe and has been present since the late 1800’s. There have been calls to remove livestock grazing from rangelands as historic grazing practices resulted in the degradation of some...
The organs that make up the gastrointestinal tract have high energy demands. Therefore, when these organs vary in mass, they should impact metabolic requirements. Mass of the rumen-reticulum organs, the organs that comprise the largest part of the gastrointestinal tract of ruminants, might vary from bulk or nutrient availability of...
We inventoried lichens in Lake Clark (LACL) and Katmai (KATM) National Parks and Preserves. We assembled the known information on lichens in these parks by combining field, herbarium, and literature studies. Our results provide baseline data on lichen occurrence that may be used in resource condition assessments, vulnerability assessments, long-term...
Intracellular pH (pH(i)) imaging is of paramount importance for life sciences. In this work, we implement the ultrafast electronic and stimulated Raman spectroscopies to unravel the fluorescence mechanism of an excitation-ratiometric pH(i) indicator in basic aqueous solution. After photoexcitation of the pH(i) indicator HPTS, a hidden charge-transfer (CT) state following...
Biosensors have become an indispensable tool set in life sciences. Among them, fluorescent protein-based biosensors have great biocompatibility and tunable emission properties but their development is largely on trial and error. To facilitate a rational design, we implement tunable femtosecond stimulated Raman spectroscopy, aided by transient absorption and quantum calculations,...
Resonance enhancement has been increasingly employed in the emergent femtosecond stimulated Raman spectroscopy (FSRS) to selectively monitor molecular structure and dynamics with improved spectral and temporal resolutions and signal-to-noise ratios. Such joint efforts by the technique- and application-oriented scientists and engineers have laid the foundation for exploiting the tunable FSRS...
We strategically modified the GFP core via chemical synthesis to make redder and brighter biomimetic fluorophores. Based on quantum calculations, solvatochromism analysis, and femtosecond Raman, we unveiled the additive effect of tuning the electronic ground and excited states, respectively, to achieve a dramatic emission redshift with a "double-donor-one-acceptor" structure.