Escherichia coli 16S-like ribosomal RNAs from
eubacterial, archaebacterial and eukaryotic organisms have
several universally conserved sequence zones which are
proposed to be single-stranded. A clear function for all
of these regions has not yet been defined. To evaluate the
roles of these sequence zones in protein synthesis,
knowledge of their...
In an attempt to study the times in the lifecycle of
Bdellovibrio bacteriovorus at which various genes are
transcribed, the patterns of messenger ribonucleic acid
(mRNA) and ribosomal ribonucleic acid (rRNA) syntheses
were examined using two different types of hybridization
systems. The hybridization system using membrane bound
deoxyribonucleic acid (DNA)...
Ribosomes are multicomponent macromolecular particles and
are essential for the survival of cells in all organisms. The ribosome's
universal function is to catalyze polypeptide synthesis through
translation of mRNA transcripts. Ribosomes from Escherichia coli,
eubacterial organisms, have a sedimentation coefficient of 70S and are
composed of 30S and 50S ribonucleoprotein...
Seed dormancy is defined as the inability of viable seeds to germinate under conditionsotherwise favorable for germination. Dormancy provides a strategy for seeds to germinateat an appropriate time. Abscisic acid (ABA) is a major hormone involved in the regulationof seed dormancy. To elucidate the molecular mechanisms of dormancy, the twoexperimental...
Ribosomes are ribonucleoprotein particles that are essential for
the process of protein synthesis in all living systems. Escherichia
coli, eubacterial organisms, have ribosomes which display a 70S
sedimentation coefficient. They are formed by the association of two
ribonucleoprotein subunits which sediment at 50S and 30S. The 30S
subunit is a...
Differential expression (DE) analysis is a key task in gene expression study, because it uncovers the association between expression levels of a gene and the covariates of interest. This dissertation pertains to two particular aspects of DE analysis—identifying stably expressed genes for count normalization and accounting for correlation between DE...
Due to a number of disadvantages associated with the use of antibodies as
affinity stationary phases, researchers have recently began to explore the use of RNA
and DNA aptamers for use as affinity stationary phases. These molecules have been
shown to be a viable choice for applications in many methods...