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Filtering by: Creator Smythe, Sharon E. Non-Academic Affiliation Oregon State University. Writing Intensive Curriculum

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

Creator:

Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Williams, David E., and Baird, William M.

Abstract:

Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...

Resource Type:

Research Paper

Full Text:

with environmental PAH mixtures Andrew Larkina,b, Lisbeth K. Siddensb, Sharon K. Kruegerc, David E