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  • Cryopreservation of in vitro-grown apical meristems

    Creator:
    Chang, Yongjian
    Abstract:
    Alternating temperature (22°C / -1°C) pretreatments were effective for increasing cold hardiness and recovery of cryopreserved meristems. Combining low temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the time required for optimal cold hardiness of meristems. Desiccation tolerance was directly correlated with increased cold hardiness and recovery of...
    Resource Type:
    Dissertation
    Full Text:
    increased cryopreserved meristem regrowth and shoot cold hardiness. However, ABA significantly shortened