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  • Nucleoprotein structure of adenovirus

    Creator:
    Corden, Jeffry Lynn
    Abstract:
    The structure of the nucleoprotein core of adenovirus has been investigated. Using a combination of biophysical techniques and nuclease digestion I have determined that the adenovirus core, as isolated with pyridine, shares several physical properties with cellular chromatin. The most notable difference is the lack of a nucleosome repeat in...
    Resource Type:
    Dissertation

  • Antigenic comparison of five bovine adenovirus type 3 isolates using a fluorescent foci assay system

    Creator:
    Liauw, Hardi Kian-hong
    Abstract:
    Four bovine adenovirus type 3 isolates, recovered from different areas in Oregon and Washington, were compared antigenically with the original prototype strain which was isolated in England. The fluorescent foci assay method was used to quantitatively establish viral titers. A serum-viral neutralization test was performed with a standard dose of...
    Resource Type:
    Masters Thesis

  • The kinetics of adenovirus DNA replication

    Creator:
    Bodnar, John W.
    Abstract:
    Methods have been developed to measure the time required to complete replication of an adenovirus DNA molecule and the time between rounds of replication of the viral genomes. These methods show that 22 minutes are required to complete replication of an adenovirus DNA molecule at 37° C and that this...
    Resource Type:
    Dissertation

  • Intramolecular and intermolecular strand hybridization during adenovirus DNA replication

    Creator:
    Xu, Fu-yun
    Abstract:
    Adenovirus initiates strand-displacement DNA replication from origins located in identical inverted terminal repetitions (ITRs). Panhandle structures, formed by base-pairing between ITRs on the displaced strands, have been proposed as replication intermediates for complementary strand synthesis. Plasmid molecules containing a single adenovirus terminal sequence and specially arranged inverted repeat sequences were...
    Resource Type:
    Dissertation

  • In vitro adenovirus DNA replication

    Creator:
    Chow, Kuan-chih W.
    Resource Type:
    Dissertation

  • Adenovirus replication in trans : a new replication pathway

    Creator:
    Ahern, Kevin
    Abstract:
    Replication of plasmid molecules containing either a left-end or a right-end adenovirus origin was investigated. When the plasmids were linearized to expose their respective adenovirus termini, mixed, and transfected with helper adenovirus DNA into the human 293 cell line, a new molecule was detected. The new molecule was the size...
    Resource Type:
    Dissertation

  • Structural requirements for adenovirus DNA replication

    Creator:
    Hu, Chin-Hwa
    Abstract:
    The adenovirus replication origins reside in the inverted terminal repetition (ITR) sequences. Replication proceeds unidirectionally by a strand-displacement mechanism from either or both origins to produce duplex daughter DNA plus a displaced parental strand. The displaced strand can be duplicated by two different routes. The cis pathway involves intramolecular hybridization...
    Resource Type:
    Dissertation

  • Adenovirus complementary strand synthesis in vivo

    Creator:
    Wang, Kai, 1955-
    Abstract:
    Replication of plasmid molecules containing a single adenovirus terminal sequence and specially arranged inverted repeat sequences were studied. When the plasmids were linearized to expose the adenovirus terminal sequence and transfected into 293 cells with helper adenovirus DNA, a larger molecule was detected. The new molecule contained adenovirus terminal sequences...
    Resource Type:
    Dissertation

  • Sequence conversion during adenovirus DNA replication

    Creator:
    Bennett, Kelly L.
    Abstract:
    The work in this thesis has provided conclusive genetic evidence that "panhandle" intermediates form during adenovirus replication. Adenovirus chromosomes lacking 51 by from their left -hand termini are infectious and capable of regenerating the missing origin sequence. Yet if an entire inverted terminal repeat is removed, the adenovirus chromosome is...
    Resource Type:
    Dissertation

  • Antigenic comparison of bovine, ovine, equine, and llama adenoviruses

    Creator:
    Yusuf, Irwandi
    Abstract:
    Fifteen adenoviruses from cattle, sheep, horses, and llamas were studied by virus neutralization to determine their degree of antigenic similarity. Prototype viruses included bovine adenoviruses species 1-8, ovine adenoviruses species 5 and 6, and equine adenovirus species 1. Unclassified viruses that were compared to the prototype viruses were isolated from...
    Resource Type:
    Masters Thesis

  • Development of an enzyme-linked immunosorbent assay for the serologic diagnosis of bovine adenovirus type 3

    Creator:
    Whipple, Margaret Jo
    Abstract:
    An enzyme-linked immunosorbent assay was developed to measure specific antibody response in bovine sera to bovine adenovirus type 3 (BA3), an etiologic agent of respiratory disease causing economic losses annualy to the cattle industry. Observed endpoint titers were determined using the intersection point from optical density values of serially diluted...
    Resource Type:
    Masters Thesis

  • Characterization of adenovirus isolated from sheep in Oregon

    Creator:
    Babar, Shakeel
    Abstract:
    Six 3 to 4 weeks old, cesarian-derived lambs were inoculated with ovine an adenovirus isolate 475N. Inoculated lambs showed moderate clinical signs of respiratory distress, conjunctivitis, and loose feces during the 10-day observation period. Virus was detected from nasal and conjunctival swabs starting on postinoculation day (PID) 2. Virus was...
    Resource Type:
    Dissertation