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Creator:: Kuo, Mei
Abstract:: Gene transfer via interspecific hybridization within Phaseolus has been limited by abnormal hybrid development and the rapid loss of hybridity in subsequent generations. The most advanced embryo development occurs in P. vulgaris x P. coccineus where mature seeds form. However, the reciprocal cross still results in abnormal embryos unable to...
Resource Type:: Dissertation
Full Text:: Interspecific Hybrids of Phaseolus Abstract approved: ^ David W.S. Mok (Professor) Gene transfer via

Creator:: Dixon, Susan C.
Abstract:: An enzyme catalyzing the formation of O-glucosylzeatin in immature embryos of P. lunatus was purified 2500 fold using ammonium sulfate precipitation followed by affinity and anion exchange chromatography. The enzyme uses trans-zeatin as substrate (Km 28 uM) but not cis-zeatin. ribosylzeatin, or dihydrozeatin. Both UDP-glucose and UDP-xylose can serve as...
Resource Type:: Dissertation
Full Text:: Metabolic Enzymes from Phaseolus Abstract approved: ^„ ^ r;~. ,, ^ - . Dr. David W. S. Mok

Creator:: Martin, Ruth C.
Abstract:: A critical balance of hormones is necessary for normal plant development. Therefore, precise mechanisms must exist to maintain proper levels of cytokinins, a group of hormones regulating cell division and differentiation. Structural modifications of the naturally occurring cytokinin, zeatin; including the oxidation, O-glycosylation and reduction of the N⁶-side chain; may...
Resource Type:: Masters Thesis
Full Text:: Reductase in Phaseolus Embryos Abstract approved: .-^^ - -'-s' —David W. S. Mok A

Creator:: Martin, Ruth C.
Abstract:: Cytokinins are a group of plant hormones mediating cell division and differentiation. Zeatin is a naturally occurring and highly active cytokinin that is rapidly metabolized in Phaseolus seeds. The metabolites and enzymes mediating the conversion have been studied extensively. Zeatin metabolic enzymes may be utilized in studying the regulation of...
Resource Type:: Dissertation
Full Text:: Phaseolus vulgaris: Immunological and Molecular Analyses Abstract approved: , ^6avid W. S. Mok

Creator:: Guo, Mei
Abstract:: Interspecific hybridization between Phaseolus vulgaris and P. coccineus results in the formation of either mature F₁ seeds or abnormal embryos, depending on the direction of the cross. However, normal as well as abnormal embryos occur in the reciprocal F₂ populations. The exact cause of abnormal embryo development is unknown. To...
Resource Type:: Masters Thesis
Full Text:: ACKNOWLEDGMENTS I wish to express my sincere gratitude to my major professor, Dr. David W. S. Mok for his

Creator:: Lee, Yun-hwa
Resource Type:: Masters Thesis
Full Text:: Metabolism in Phaseolus Embryos Abstract approved: .__ Dr. 'David W. S. Mok The metabolism of [8-14c

Creator:: Shii, Chou Tou
Abstract:: Hybrids with abnormal development were obtained when particular genotypes of Phaseolus vulgaris L. were crossed. The phenotypic aberrations of the F₁s included retarded growth, chlorosis of the trifoliate leaves and the formation of adventitious roots on the hypocotyls. The expression of the abnormalities was dependent on the temperature. At high...
Resource Type:: Dissertation
Full Text:: Phaseolus vulgaris L. Abstract approved: __ Dr. David W. S. Mok Hybrids with abnormal

Creator:: Rabakoarihanta, Aimee
Abstract:: Interspecific hybridizations between P. vulgaris and P. acutifolius and between P. vulgaris and P. lunatus were investigated. Prefertilization events were similar in inter- and intra-specific crosses. Fertilization was completed in all crosses and the time of fertilization was dependent on the maternal parent, reflecting differences in the time of maturation...
Resource Type:: Dissertation
Full Text:: / - ^ - - r ^ Dr. David W. S. Mok Interspecific hybridizations between P_. vulgaris and P_

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