Gene synthesis attempts to assemble user-defined DNA sequences with base-level precision. Verifying the sequences of construction intermediates and the final product of a gene synthesis project is a critical part of the workflow, yet one that has received the least attention. Sequence validation is equally important for other kinds of...
For the past six years in which overwintering mortality of honey bee colonies has been surveyed in the USA, estimates of colony loss have
fluctuated around one-third of the national population. Here we report on the losses for the 2012-2013 seasons. We collected data from 6,482
US beekeepers (6,114 backyard,...
This study records the fifth consecutive year that winter losses of managed honey bee (Apis mellifera) colonies in the USA have been around 30%. In April 2011, a total of 5,441 US beekeepers (an estimated 11% of total US beekeepers) responded to a survey conducted by the Bee Informed Partnership....
Estimates of winter loss for managed honey bee (Apis mellifera) colonies are an important measure of honey bee health and productivity. We used data from 5,500 US beekeepers (5,244 backyard, 189 sideline and 67 commercial beekeepers) who responded to the April 2012 Bee Informed Partnership Winter Colony Loss Survey and...
Historic and current use pesticides (HUPs and CUPs), with respect to use in the United States and Canada, were identified in trans-Pacific and regional air masses at Mt. Bachelor Observatory (MBO), a remote high elevation mountain in Oregon’s Cascade Range located in the United States, during the sampling period of...
As part of the Sagebrush Steppe Treatment Evaluation Project (SageSTEP), butterflies were surveyed pre-treatment and up to four years post-treatment at 16 widely distributed sagebrush steppe sites in the interior West. Butterfly populations and communities were analyzed in response to treatments (prescribed fire, mechanical, herbicide) designed to restore sagebrush steppe...
Dietary restriction (DR) is the gold standard intervention used to delay aging, and much recent research has focused on
the identification of possible DR mimetics. Energy sensing pathways, including insulin/IGF1 signaling, sirtuins, and
mammalian Target of Rapamycin (mTOR), have been proposed as pathways involved in the antiaging actions of DR,...
Studies on hydromineral balance in fishes frequently employ measurements of electrolytes following euthanasia. We tested the effects of fresh- or salt-water euthanasia baths of tricaine mesylate (MS-222) on plasma magnesium (Mg²⁺) and sodium (Na⁺) ions, cortisol and osmolality in fish exposed to saltwater challenges, and the ion and steroid hormone...
The discovery on Mars of recurring slope lineae (RSL), thought to represent seasonal brines, has sparked interest in analogous environments on Earth. We report on new studies of Don Juan Pond (DJP), which exists at the upper limit of ephemeral water in the McMurdo Dry Valleys (MDV) of Antarctica, and...
INTRODUCTION: Tissue engineering is a new methodology for addressing meniscal
injury or loss. Synovium may be an ideal source of cells for in vitro meniscal fibrocartilage
formation, however, favorable in vitro culture conditions for synovium must be
established in order to achieve this goal. The objective of this study was...