Solvent extraction is an integral chemical and biochemical separation process that is drastically intensified in microfluidic systems. A novel high-throughput micro-separator was additively manufactured for the intensification of liquid–liquid separation. The micro-posts array within the flow plate generated a capillary pressure gradient on the non-wetting organic phase, enabling continuous, membrane-free,...
Wildfire is an annual threat for many rural communities in the Pacific Northwest region of the United States. In some severe events, evacuation is one potential course of action to gain safety from an advancing wildfire. Since most evacuations occur in a personal vehicle along the surrounding road network, the...
Two-photon, planar laser-induced fluorescence (PLIF) of carbon-monoxide (CO) and two-dimensional thermometry employing two-color, hydroxyl radical (OH) PLIF are used to characterize atmospheric-pressure inverse diffusion flames. These flames are important tools to aid the understanding of secondary reaction zones that may form in gas turbine engines when film-cooling air reacts with...
Aims:
To design and validate a colorimetric loop-mediated isothermal amplification assay for rapid detection of Phytophthora infestans DNA.
Methods and Results:
Two sets of loop-mediated isothermal amplification (LAMP) primers were designed and evaluated for their sensitivity and specificity for P. infestans. ITSII primers targeted a portion of the internal transcribed...
The Juneau Wetland Management Plan (JWMP), Volume 1, provides results of wetlands mapping and wetland functional assessments for currently undeveloped parcels within the City and Borough of Juneau (CBJ). The study areas were selected for their potential for future development based on low elevation and proximity to existing infrastructure. During...
Management of grape powdery mildew (Erysiphe necator) and other polycyclic diseases often relies on calendar-based pesticide application schedules that assume the presence of inoculum. An inexpensive, loop-mediated isothermal amplification (LAMP) assay was designed to quickly detect airborne inoculum of E. necator to determine when to initiate a fungicide application programme....
Fuel accumulation and climate shifts are predicted to increase the frequency of high-severity fires in pon-derosa pine (Pinus ponderosa) forests of central Oregon. The combustion of fuels containing large downedwood can result in intense soil heating, alteration of soil properties, and mortality of microbes. Previousstudies show ectomycorrhizal fungi (EMF) improve...
The genus Phytophthora contains some of the most notorious plant pathogens affecting nursery crops. Given the recent emergence of the sudden oak death pathogen Phytophthora ramorum, particularly in association with Rhododendron spp., characterization of Phytophthora communities associated with this host in nursery environments is prudent. Many taxa may present symptoms...
OBJECTIVES: Secondary caries is the most common reason for composite restoration replacement and usually forms between dentin and the filling. The objective of this study was to investigate the combined effect of cyclic loading and bacterial exposure on bacterial penetration into gaps at the interface between dentin and resin composite...