Plasmid transformation of chlamydiae has created new opportunities to investigate host–microbe interactions during chlamydial infections; however, there are still limitations. Plasmid transformation requires a replicon derived from the native Chlamydia plasmid, and these transformations are species-specific. We explored the utility of a broad host-range plasmid, pBBR1MCS-4, to transform chlamydiae, with...
The role of volumetric energy density on the microstructural evolution, texture and mechanical properties of 304L stainless steel parts additively manufactured via selective laser melting process is investigated. 304L is chosen because it is a potential candidate to be used as a matrix in a metal matrix composite with nanoparticles...
Data from three micronetworks with eddy correlation data and three additional micronetworks without eddy correlation data are analyzed to study the spatial variability of surface air temperature in complex terrain. A simple similarity relationship is constructed to relate the spatial variation of air temperature along the slope to the thermal...
Lithospheric cracking by remotely applied stresses or thermoelastic stresses has been suggested to be the mechanism responsible for the formation of intraplate volcanic ridges in the Pacific that clearly do not form above fixed hot spots. As part of the Gravity Lineations Intraplate Melting Petrology and Seismic Expedition (GLIMPSE) project...
Electromagnetic wave propagation in complex dispersive media is governed by the time dependent Maxwell’s equations coupled to equations that describe the evolution of the induced macroscopic polarization. We consider “polydispersive” materials represented by distributions of dielectric parameters in a polarization model. The work focuses on a novel computational framework for...
Solar thermochemical energy storage has enormous potential for enabling cost-effective concentrated solar power (CSP). A thermochemical storage system based on a SrO/SrCO₃ carbonation cycle offers the ability to store and release high temperature (≈1200 °C) heat. The energy density of SrCO₃/SrO systems supported by zirconia-based sintering inhibitors was investigated for 15...
In this paper, the methodology of the directed relation graph with error propagation and sensitivity analysis (DRGEPSA), proposed by Niemeyer et al. [Combustion and Flame 157 2010) 1760–1770], and its differences to the original directed relation graph method are described. Using DRGEPSA, the detailed mechanism of ethylene containing 71 species...
The complement system is an innate immune pathway that in vertebrates, is responsible for initial recognition and ultimately phagocytosis and destruction of microbes. Several complement molecules including C3, Factor B, and mannose binding lectin associated serine proteases (MASP) have been characterized in invertebrates and while most studies have focused on...
Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing sporulation remain unclear. Bile salts...
Bulk metallic glasses (BMGs) have been reported to have large variability in mode I fracture toughness. In this study, alternating soft and hard regions were created in a Zr₅₂.₅Ti₅Cu₁₈Ni₁₄.₅Al₁₀ (at%) BMG via mechanical imprinting at room temperature. While only 50% of as-cast samples demonstrated plastic deformation during mode I fracture...