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Creator:: Dolja, Valerian V. and Koonin, Eugene V.
Abstract:: Important progress in understanding replication, interactions with host plants, and evolution of closteroviruses enabled engineering of several vectors for gene expression and virus-induced gene silencing. Due to the broad host range of closteroviruses, these vectors expanded vector applicability to include important woody plants such as citrus and grapevine. Furthermore, large...
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Full Text:: : Valerian V. Dolja, Department of Botany and Plant Pathology, Oregon State University, Cordley Hall 2082

Creator:: Koonin, Eugene V. and Dolja, Valerian V.
Abstract:: Viruses and/or virus-like selfish elements are associated with all cellular life forms and are the most abundant biological entities on Earth, with the number of virus particles in many environments exceeding the number of cells by one to two orders of magnitude. The genetic diversity of viruses is commensurately enormous...
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Full Text:: Valerian V Dolja2 Available online at www.sciencedirect.com Viruses and/or virus-like selfish elements are

Creator:: Koonin, Eugene V. and Dolja, Valerian V.
Abstract:: Viruses were defined as one of the two principal types of organisms in the biosphere, namely, as capsid-encoding organisms in contrast to ribosome-encoding organisms, i.e., all cellular life forms. Structurally similar, apparently homologous capsids are present in a huge variety of icosahedral viruses that infect bacteria, archaea, and eukaryotes. These...
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Full Text:: Elements Koonin, E. V., & Dolja, V. V. (2014). Virus World as an Evolutionary Network of Viruses and

Creator:: Koonin, Eugene V., Dolja, Valerian V., and Krupovic, Mart
Abstract:: Viruses and other selfish genetic elements are dominant entities in the biosphere, with respect to both physical abundance and genetic diversity. Various selfish elements parasitize on all cellular life forms. The relative abundances of different classes of viruses are dramatically different between prokaryotes and eukaryotes. In prokaryotes, the great majority...
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Full Text:: viruses of eukaryotes: The ultimate modularity Eugene V. Koonin a,n, Valerian V. Dolja b, Mart Krupovic c

Creator:: Peremyslov, Valera V., Cole, Rex A., Fowler, John E., and Dolja, Valerian V.
Abstract:: Using genetic approaches, particle image velocimetry and an inert tracer of cytoplasmic streaming, we have made a mechanistic connection between the motor proteins (myosins XI), cargo transported by these motors (distinct endomembrane compartment defined by membrane-anchored MyoB receptors) and the process of cytoplasmic streaming in plant cells. It is shown...
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Full Text:: Expansion and Plant Development Valera V. Peremyslov, Rex A. Cole, John E. Fowler, Valerian V. Dolja

Creator:: Krupovic, Mart, Dolja, Valerian V., and Koonin, Eugene V.
Abstract:: Plant viruses of the recently recognized family Amalgaviridae have monopartite double-stranded (ds) RNA genomes and encode two proteins: an RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP). Whereas the RdRp of amalgaviruses has been found to be most closely related to the RdRps of dsRNA viruses of the...
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Creator:: Amari, Khalid, Di Donato, Martin, Dolja, Valerian V., and Heinlein, Manfred
Abstract:: Viruses are obligatory parasites that depend on host cellular factors for their replication as well as for their local and systemic movement to establish infection. Although myosin motors are thought to contribute to plant virus infection, their exact roles in the specific infection steps have not been addressed. Here we...
Resource Type:: Article

Creator:: Peremyslov, Valera V., Morgun, Eva A., Kurth, Elizabeth G., Makarova, Kira S., Koonin, Eugene V., and Dolja, Valerian V.
Abstract:: To characterize the mechanism through which myosin XI-K attaches to its principal endomembrane cargo, a yeast two-hybrid library of Arabidopsis thaliana cDNAs was screened using the myosin cargo binding domain as bait. This screen identified two previously uncharacterized transmembrane proteins (hereinafter myosin binding proteins or MyoB1/2) that share a myosin...
Resource Type:: Article
Full Text:: (www.plantcell.org) is: Valerian V. Dolja (doljav@ science.oregonstate.edu). W Online version contains Web-only data

Creator:: Kurth, Elizabeth G., Peremyslov, Valera V., Prokhnevsky, Alexey I., Kasschau, Kristin D., Miller, Marilyn, Carrington, James C., and Dolja, Valerian V.
Abstract:: The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
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Full Text:: ):6002. DOI: 10.1128/JVI.00436-12. J. Virol. Carrington and Valerian V. Dolja Prokhnevsky, Kristin D

Creator:: Peremyslov, Valera V., Mockler, Todd C., Filichkin, Sergei A., Fox, Samuel E., Jaiswal, Pankaj, Makarova, Kira S., Koonin, Eugene V., and Dolja, Valerian V.
Abstract:: Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin organization, and cell and plant growth. Due to the large size of myosin gene families, knowledge of these molecular motors remains patchy. Using deep transcriptome sequencing and bioinformatics, we systematically investigated myosin...
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