Index Catalog // ScholarsArchive@OSU

Excited State Structural Events of a Dual-Emission Fluorescent Protein Biosensor for Ca²⁺ Imaging Studied by Femtosecond Stimulated Raman Spectroscopy

Creator:: Wang, Yanli, Tang, Longteng, Liu, Weimin, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Resource Type:: Article
Full Text:: with a <250 fs time constant. Upon Ca2+ binding, the chromophore adopts a more twisted conformation

Five million years of compositionally diverse, episodic volcanism : construction of Davidson Seamount atop an abandoned spreading center

Creator:: Clague, D. A., Paduan, J. B., Duncan, Robert A., Huard, J. J., Davis, A. S., Castillo, P. R., Lonsdale, P., and DeVogelaere
Abstract:: Davidson Seamount, a volcano located about 80 km off the central California coast, has a volume of ~320 km³ and consists of a series of parallel ridges serrated with steep cones. Davidson was sampled and its morphology observed during 27 ROV Tiburon dives. During those dives, 286 samples of lava,...
Resource Type:: Article
Full Text:: 80 km off the central California coast, has a volume of �320 km3 and consists of a series of parallel

Excited-State Proton Transfer of Photoexcited Pyranine in Water Observed by Femtosecond Stimulated Raman Spectroscopy

Creator:: Han, Fangyuan, Liu, Weimin, and Fang, Chong
Abstract:: We use femtosecond stimulated Raman spectroscopy (FSRS) to illuminate the choreography of intermolecular excited-state proton transfer (ESPT) of photoacid pyranine (8-hydroxypyrene-1,3,6-trisulfonic acid, HPTS) in water. The multidimensional reaction coordinate responsible for photoacidity is revealed to involve sequential activation of characteristic skeletal motions during the ca. 1 ps preparation stage preceding...
Resource Type:: Article
Full Text:: cm -1 mode, shows a rising exponential time constant of 320 fs and a decay constant of 540 fs

Tunable sideband laser from cascaded four-wave mixing in thin glass for ultra-broadband femtosecond stimulated Raman spectroscopy

Creator:: Zhu, Liangdong, Liu, Weimin, and Fang, Chong
Abstract:: We demonstrate the generation of broadband up-converted multicolor array (BUMA) in a thin BK7 glass slide using two noncollinear weak near-IR laser pulses with various crossing angles. The BUMA signal arises from cubic nonlinear χ⁽³⁾:χ⁽³⁾ processes via cascaded four-wave mixing of the two incident beams. Broad and continuous tunability of...
Resource Type:: Article
Full Text:: - seconds (fs) to picoseconds (ps), the laser pulses utilized must have shorter duration than the response

Terahertz-wave generation in quasi-phase-matched GaAs

Creator:: Vodopyanov, K. L., Fejer, M. M., Yu, X., Harris, J. S., Lee, Yun-Shik, Hurlbut, Walter C., Kozlov, V. G., Bliss, D., and Lynch, C.
Abstract:: The authors demonstrate an efficient room temperature source of terahertz radiation using femtosecond laser pulses as a pump and GaAs structures with periodically inverted crystalline orientation, such as diffusion-bonded stacked GaAs and epitaxially grown orientation-patterned GaAs, as a nonlinear optical medium. By changing the GaAs orientation-reversal period (504–1277 μm), or...
Resource Type:: Article
Full Text:: rectification in LiNbO3, in a noncollinear geometry, with 150 fs 300–500 �J optical pump pulses at 800 nm with a

Excited state structural dynamics of a dual-emission calmodulingreen fluorescent protein sensor for calcium ion imaging

Creator:: Oscar, Breland G., Liu, Weimin, Zhao, Yongxin, Tang, Longteng, Wang, Yanli, Campbell, Robert E., and Fang, Chong
Abstract:: Fluorescent proteins (FPs) have played a pivotal role in bioimaging and advancing biomedicine. The versatile fluorescence from engineered, genetically encodable FP variants greatly enhances cellular imaging capabilities, which are dictated by excited state structural dynamics of the embedded chromophore inside the protein pocket. Visualization of the molecular choreography of the...
Resource Type:: Article
Full Text:: strongest A* mode intensity at 1180 cm-1 (Fig. 3A) shows an ultrafast rising component within the 140 fs

Rhizopogon togasawariana sp. nov., the first report of Rhizopogon associated with an Asian species of Pseudotsuga

Creator:: Mujic, Alija B., Hosaka, Kentaro, and Spatafora, Joseph W.
Abstract:: Rhizopogon subgenus Villosuli are the only members of the genus known to form an ectomycorrhizal relationship exclusively with Pseudotsuga. The specificity of this host relationship is unusual in that Rhizopogon is broadly associated with several tree genera within the Pinaceae and relationships with a host genus are typically distributed across...
Resource Type:: Article
Full Text:: numbers FS-91, FS -95, FS-214, FS-151, FS-164 and FS-225. New sequences generated in this study and

Nature and Extent of the Deep Biosphere

Creator:: Colwell, Frederick S. and D’Hondt, Steven
Abstract:: In the last three decades we have learned a great deal about microbes in subsurface environments. Once, these habitats were rarely examined, perhaps because so much of the life that we are concerned with exists at the surface and seems to pace its metabolic and evolutionary rhythms with the overt...
Resource Type:: Article

High-field terahertz response of graphene

Creator:: Paul, M. J., Chang, Y. C., Thompson, Z. J., Stickel, A., Wardini, J., Choi, H., Minot, E. D., Norris, T. B., and Lee, Yun-Shik
Abstract:: We investigate the response of multi-layer epitaxial graphene and chemical vapor deposition (CVD)-grown single-layer graphene to strong terahertz (THz) fields. Contrary to theoretical predictions of strong nonlinear response, the transmitted fields exhibit no harmonic generation, indicating that the nonlinear response is limited by fast electron thermalization due to carrier-carrier scattering....
Resource Type:: Article
Full Text:: system (lambda cubed laser system at University of Michigan) produces 30 fs, 3mJ pulses [35]. The beam is

Marine bird aggregations associated with the tidally-driven plume and plume fronts of the Columbia River

Creator:: Zamon, Jeannette E., Phillips, Elizabeth M., and Guy, Troy J.
Abstract:: Freshwater discharge from large rivers into the coastal ocean creates tidally-driven frontal systems known to enhance mixing, primary production, and secondary production. Many authors suggest that tidal plume fronts increase energy flow to fish-eating predators by attracting planktivorous fishes to feed on plankton aggregated by the fronts. However, few studies...
Resource Type:: Article
Full Text:: marine waters (from north to south: Father and Son-FS, La Push-LP, Queets River-QR, Grays Harbor-GH

ScholarsArchive@OSU

Excited State Structural Events of a Dual-Emission Fluorescent Protein Biosensor for Ca²⁺ Imaging Studied by Femtosecond Stimulated Raman Spectroscopy

Five million years of compositionally diverse, episodic volcanism : construction of Davidson Seamount atop an abandoned spreading center

Excited-State Proton Transfer of Photoexcited Pyranine in Water Observed by Femtosecond Stimulated Raman Spectroscopy

Tunable sideband laser from cascaded four-wave mixing in thin glass for ultra-broadband femtosecond stimulated Raman spectroscopy

Terahertz-wave generation in quasi-phase-matched GaAs

Excited state structural dynamics of a dual-emission calmodulingreen fluorescent protein sensor for calcium ion imaging

Rhizopogon togasawariana sp. nov., the first report of Rhizopogon associated with an Asian species of Pseudotsuga

Nature and Extent of the Deep Biosphere

High-field terahertz response of graphene

Marine bird aggregations associated with the tidally-driven plume and plume fronts of the Columbia River

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