Real-time PCR assays using locked nucleic acid (LNA) probes and high resolution melt (HRM) analysis were developed for molecular differentiation of Pseudoperonospora cubensis and P. humuli, causal agents of cucurbit and hop downy mildew, respectively. The assays were based on a previously identified single nucleotide polymorphism (SNP) in the cytochrome...
Discerning the intricacies of mating systems in butterflies can be difficult,
particularly when multiple mating strategies are employed and are cryptic and not
exclusive. We observed the behavior and habitat use of 113 male Taylor’s
checkerspot butterflies (Euphydryas editha taylori). We confirmed that two distinct
mating strategies were exhibited; patrolling...
Spatially isolated patches of soil located in Taylor Valley, McMurdo Dry Valleys, Antarctica, are sites of elevated salt content and soil moisture. During Antarctic spring, in the absence of snow melt, visibly wet (reduced albedo) patches of soil are present at the surface. The soil pore fluids are hypersaline and...
Marine calcifiers as a plankton functional type (PFT) are a crucial part of the global carbon cycle, being responsible for much of the carbon export to the deep ocean entering via biological pathways. Deep ocean carbon export through calcifiers is controlled by physiological, ecological and biogeochemical factors. This paper describes...
Addressing the ongoing decline of Pacific Lamprey Entosphenus tridentatus across its range along the west coast
of North America requires an understanding of all life history phases. Currently, spawning surveys (redd counts)
are a common tool used to monitor returning adult salmonids, but the methods are in their infancy for...
The advancement of Coastal Ocean Forecasting Systems (COFS) requires the support of continuous scientific progress addressing: (a) the primary mechanisms driving coastal circulation; (b) methods to achieve fully integrated coastal systems (observations and models), that are dynamically embedded in larger scale systems; and (c) methods to adequately represent air-sea and...
The Pacific lamprey (Entosphenus tridentatus) is in decline in the Columbia River Basin, and translocating adult lamprey to bypass difficult migration corridors has been implemented since 2000. We describe and report results from two current translocation programs, provide context for use of translocation, and discuss potential benefits, risks, and uncertainties....
Exposure to arsenic early in life has been associated with increased risk of several chronic diseases and is believed to alter epigenetic programming in utero. In the present study, we evaluate the epigenome-wide association of arsenic exposure in utero and DNA methylation in placenta (n=37), umbilical artery (n=45) and human...
This perspectives paper uses the seminal Goodchild (1992) article as a lens through which to review and reflect upon several longstanding issues that have influenced the field of geographic information science in the past and will continue to be important at least into the next decade. Under the category of...
Several evolutionarily significant units (ESUs) of Columbia River basin Chinook Salmon Oncorhynchus tshawytscha and Coho Salmon O. kisutch are listed as threatened or endangered under the U.S. Endangered Species Act. Yet little is known about the spatial and temporal distributions of these ESUs immediately following ocean entry, when year-class success...