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Quorum sensing gene regulation in Pseudomonas aeruginosa

Creator:: Gupta, Rashmi
Abstract:: Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
Resource Type:: Dissertation
Full Text:: Quorum Sensing Gene Regulation in Pseudomonas aeruginosa by Rashmi Gupta A

Characterization of the interaction between Mycobacterium avium subsp. paratuberculosis and bovine epithelial cells in culture and identification of invasion-associated genes by transposon mutagenesis

Creator:: Patel, Dilip
Abstract:: Mycobacterium avium subsp. paratuberculosis (MAP) is a cause of Johne's disease (JD) in cattle and other ruminants. MAP infection in the bovine host is not well characterized. It is assumed that crossing the bovine intestinal mucosa is important for MAP to establish infection. MAP's ability to infect bovine epithelial cells...
Resource Type:: Dissertation
Full Text:: epithelial cells, can be carried out and association of a bacterial gene with a particular phenotype can be

Physiological, proteomic, and whole-genome transcriptional responses of Nitrosomonas europaea to heavy metals (Zn²⁺,Cd²⁺, Cu²⁺, and Hg²⁺) and cyanide : identification of heavy metals and cyanide stress response genes

Creator:: Park, Sunhwa
Abstract:: Nitrosomonas europaea (ATCC19718) participates in the biological removal of nitrogen from wastewater by oxidizing ammonia to nitrite, the first step in nitrification. This bacterium is sensitive to heavy metals (Zn²⁺, Cd²⁺, Cu²⁺, and Hg²⁺) and cyanide, compounds commonly encountered in wastewater treatment plants. Therefore, we characterized physiological and transcriptional responses...
Resource Type:: Dissertation
Full Text:: showed down regulation. A gene cluster that included moeZ (NE2353), encoding a rhodanese homologue

An investigation of carbon and nitrogen metabolism through a genomic analysis of the genus Nitrobacter

Creator:: Starkenburg, Shawn R.
Abstract:: The chemolithoautotrophic nitrite oxidizing bacteria (NOB) participate in the biogeochemical cycling of nitrogen by catalyzing and conserving energy from the oxidation of nitrite (NO₂-) to nitrate (NO₃-) via a nitrite oxidoreductase (NXR). The main objective of this work was to comparatively annotate and analyze the genome sequences of Nitrobacter winogradskyi...
Resource Type:: Dissertation
Full Text:: revealed that the chromosome contained an operon encoding a gene (Nwin_2648) that encoded a putative nirK

Characterization of the genomic islands from tetracycline-resistant Chlamydia suis

Creator:: Dugan, Jae
Abstract:: Tetracycline (Tet)-resistant strains of Chlamydia suis were isolated from swine farms in the Midwest. The isolation of the resistant strains was significant because Tet is an antibiotic used to treat infections by veterinarians and doctors. One of the tetracycline (Tet)-resistant strains, R19, was able to survive in Tet up to...
Resource Type:: Dissertation
Full Text:: resistant to Tet because of mutations in the ribosomal RNA gene. This is, however, not a common

Molecular basis for Clostridium perfringens spore resistance to heat and other environmental stress factors

Creator:: Raju, Deepa
Abstract:: Clostridium perfringens type A isolates producing enterotoxin (CPE) are an important cause of both food poisoning (FP) and non food borne gastrointestinal diseases (NFBGID) in both humans and animals. C. perfringens type A food poisoning is caused by isolates carrying the CPE encoding gene (cpe) on the chromosome while the...
Resource Type:: Dissertation
Full Text:: the CPE encoding gene (cpe) on the chromosome while the NFBGID isolates carry cpe on a large plasmid

Molecular and genetic assessment of selected antiporters and methyl-accepting chemotaxis proteins in Vibrio cholerae

Creator:: Quinn, Matthew J.
Abstract:: The pathogen Vibrio cholerae uses cations as a primary currency of virulence and environmental persistence, using gradients of those cations to move, acquire nutrients, and control virulence gene expression. An understanding of the overlapping roles of bioenergetics and chemotaxis in the virulence and environmental survival of V. cholerae issues from...
Resource Type:: Dissertation
Full Text:: socioeconomic conditions prevented an accurate epidemiological assessment (Nelson, 2009). A post-conflict

Understanding chlamydial biology through genome sequencing and comparative genomics

Creator:: Jeffrey, Brendan
Abstract:: The human pathogen Chlamydia trachomatis displays different phenotypes between serovars both in vivo and in vitro. The study of chlamydial biology has been hampered by the lack of a tractable genetic system. Here, chlamydial isolates with known phenotypes as well as strains generated though recombination were fully sequenced to address...
Resource Type:: Dissertation
Full Text:: absence of a useful genetic system has severely limited the ability for definitive study of gene and

Genomic-assisted determination of the natural nutrient requirements of the cosmopolitan marine bacterium 'Candidatus Pelagibacter ubique'

Creator:: Tripp, H. James
Abstract:: To identify factors limiting 'Candidatus Pelagibacter ubique' maximum cell density and growth rate in pure culture on seawater, the genome sequence of 'Cand. P. ubique' was analyzed, culturing experiments with organic and inorganic nutrient additions were made, and radiotracer uptake experiments were performed. The genome was sequenced, custom data mining...
Resource Type:: Dissertation
Full Text:: . 3. A novel glycine-activated riboswitch was found on the malate synthase gene. These three

Prokaryotes associated with marine crust

Creator:: Mason, Olivia Underwood
Abstract:: Oceanic crust covers nearly 70% of the Earth's surface, of which, the upper, sediment layer is estimated to harbor substantial microbial biomass. Marine crust; however, extends several kilometers beyond this surficial layer, and includes the basalt and gabbro layers. In particular, the basalt layer has high permeabilities which allows for...
Resource Type:: Dissertation
Full Text:: . Cloning and sequencing of 16S rDNA from basalt and gabbro samples revealed that a disparate microbial

ScholarsArchive@OSU

Quorum sensing gene regulation in Pseudomonas aeruginosa

Characterization of the interaction between Mycobacterium avium subsp. paratuberculosis and bovine epithelial cells in culture and identification of invasion-associated genes by transposon mutagenesis

Physiological, proteomic, and whole-genome transcriptional responses of Nitrosomonas europaea to heavy metals (Zn²⁺,Cd²⁺, Cu²⁺, and Hg²⁺) and cyanide : identification of heavy metals and cyanide stress response genes

An investigation of carbon and nitrogen metabolism through a genomic analysis of the genus Nitrobacter

Characterization of the genomic islands from tetracycline-resistant Chlamydia suis

Molecular basis for Clostridium perfringens spore resistance to heat and other environmental stress factors

Molecular and genetic assessment of selected antiporters and methyl-accepting chemotaxis proteins in Vibrio cholerae

Understanding chlamydial biology through genome sequencing and comparative genomics

Genomic-assisted determination of the natural nutrient requirements of the cosmopolitan marine bacterium 'Candidatus Pelagibacter ubique'

Prokaryotes associated with marine crust

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