Index Catalog // ScholarsArchive@OSU

Physical structure and reconstitution of chromatin core particles

Creator:: Tatchell, Kelly George
Abstract:: The nucleosome appears to be the basic structural unit of the eukaryotic chromosome. The usual nucleosome preparation, isolated from a micrococcal nuclease digest of nuclei, is heterogenous in DNA length and protein composition with some particles containing spacer DNA and associated protein. This heterogeneity complicated physical and reconstitution studies. As...
Resource Type:: Dissertation
Full Text:: at 60°C in 1 mM cacodylate. The rest of the DNA, approximately 100 base pairs, denature

On the location of the linker histones and the linker DNA in the 30 nm fiber of chromatin

Creator:: Leuba, Sanford H.
Abstract:: Understanding the structure of the 30 nm fiber in chromatin is relevant to understanding eukaryotic replication and transcription. The major controversy among the models of the fiber concerns the disposition of the linker DNA, the DNA between adjacent nucleosomes, and the location of the linker histones. To determine if the...
Resource Type:: Dissertation
Full Text:: C- terminal tails of the linker histones in chromatin (Bohm and Crane-Robinson, 1984). Chymotrypsin

Binding and assembly of H5 (and the globular domain of H5) onto DNA

Creator:: Carter, George John
Abstract:: In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding affinity for DNA than the isolated globular domain (GH5), supporting the importance of the...
Resource Type:: Dissertation
Full Text:: with chymotrypsin, displayed highly uniform contacts that appeared to involve the C-terminal four

Physical studies of the yeast inner histones

Creator:: Mardian, James Kenneth Walker
Abstract:: In this work, the four yeast inner histones were prepared by the use of consecutive slab gel fractionation. The use of this technique allowed the first complete fractionation and rigorous identification of the four yeast inner histones, and made possible a study of their complexing behavior. Variants and adjuncts of...
Resource Type:: Dissertation
Full Text:: January 20, 1978 Commencement June 1978 APPROVED: Redacted for Privacy Pressor of Biophysics c/ in

Physical properties of calf thymus H1 histones : conformational changes and interactions with two nonhistone chromosomal proteins

Creator:: Smerdon, Michael John
Abstract:: This work represents the first study of the conformational changes in histone H1 subfractions as well as the interactions of these subfractions with two nonhistone chromosomal (NHC) proteins. Calf thymus Hi was fractionated by the method of Kincade and Cole (1966a) using a very shallow guanidinium chloride gradient. A possible...
Resource Type:: Dissertation
Full Text:: Changes During pH Titrations 39 Conformational Changes at Neutral pH, Induced by Salt Addition 44 Part C

Structure and function of chromatin : studies at the nucleosome and nuclear matrix levels

Creator:: Shih, Ah Shing
Abstract:: Several levels of eukaryotic chromatin structure have been observed: the nucleosome, the 10 nm and 30 nm fibers and loop domains, apparently attached to the nuclear matrix. In this research, the structure and function of chromatin at two of these levels was investigated, with studies on both nucleosome positioning and...
Resource Type:: Dissertation
Full Text:: Biochemistry and Biophysics Redacted for privacy Dean of Gradua C School c3 Date thesis is presented Typed

Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

Creator:: Dong, Feng
Abstract:: By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable...
Resource Type:: Dissertation
Full Text:: Solution Stability, Dissociation and Reassociation 3 C. Salt-dependent Nucleosome Conformational

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Creator:: Weber, Shane Crawford
Abstract:: I examined, in three separate studies, the active chromatin structure of the yeast, Saccharomyces cerevisiae. Yeast contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted by 0.25...
Resource Type:: Dissertation
Full Text:: extracted either from freshly prepared chromatin or from chromatin first frozen overnight at -80°C in 2.0

Chromatin structure : possible regulatory role in gene expression

Creator:: Herman, Timothy Merle
Abstract:: Chromatin DNA sequences intimately associated with the "core" of the basic, repeating chromatin subunit are shown to be relatively resistant to digestion by micrococcal nuclease. Chromatin DNA sequences lying between adjacent chromatin subunits, as well as DNA sequences in regions of chromatin which are free of chromatin subunits, are subject...
Resource Type:: Dissertation
Full Text:: 180 base pairs. c:) 11 Axel et al. (1973a) and Cedar and Felsenfeld (1973) has shown that the

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

Creator:: Brown, D. W. (David W.), 1937-
Abstract:: Time-resolved fluorescence spectroscopy was used to monitor the effects of varying ionic strength on nucleosome core particle structure. Two main methods were used in these studies. First, the fluorescence anisotropy decay of bound ethidium was measured and was shown to reflect the rotational tumbling of the core particle through solution,...
Resource Type:: Dissertation
Full Text:: Biophysics Dean of Graduate c ool Date thesis is presented 15 September 1992 Redacted for Privacy

The binding of ethidium bromide to chromatin : model for carcinogen interactions

Creator:: McMurray, Cynthia T.
Abstract:: Five forms of chromatin, which represent the in vivo folded forms of nucleic acid, were isolated and used as the binding substrate for model intercalating compound, ethidium bromide. For all forms of chromatin, the affinity, location and structural effects of binding were examined. On the level of the nucleosome, the...
Resource Type:: Dissertation
Full Text:: 19 2. Chicken Erythrocyte DNA 19 C. 31P-NMR Studies 19 D. H 1 Nuclear Magnetic Resonance 20 E

ScholarsArchive@OSU

Physical structure and reconstitution of chromatin core particles

On the location of the linker histones and the linker DNA in the 30 nm fiber of chromatin

Binding and assembly of H5 (and the globular domain of H5) onto DNA

Physical studies of the yeast inner histones

Physical properties of calf thymus H1 histones : conformational changes and interactions with two nonhistone chromosomal proteins

Structure and function of chromatin : studies at the nucleosome and nuclear matrix levels

Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Chromatin structure : possible regulatory role in gene expression

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

The binding of ethidium bromide to chromatin : model for carcinogen interactions

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