Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
L. C. Dunn (1893-1974) spent most of his scientific career conducting research in
developmental genetics as a member of the Zoology Department at Columbia
University in the City of New York. He had an accomplished scientific career
researching mutations in mice, which earned him respect from other geneticists and
scientists....
Research on the population genetics of microbial organisms requires the use of specialized analyses designed for clonal organisms to avoid violating the assumptions of traditional population genetic models. The tools necessary for performing these analyses existed as a set of unrelated software with non-overlapping capabilities and did not cover all...
A liquid crystal thermographic technique was developed
for application to several problems in clinical
medicine and physiology. The procedure was inexpensive,
rapid, simple, versatile and acceptable to patients.
Investigators in the past have used cholesteric liquid
crystals for thermography but their use was limited to
research in the medical field...
Bromoalkoxylation of dihydropyran with bromine and 1-borneol, followed by dehydrobromination and epoxidation, afforded trans-2-1- bornyloxy-3,4-epoxytetrahydropyran as a major product. This was treated with sodium phenylselenide to give a 1:1 mixture of (2R,3R,4R)- 2-1-bornyloxy-3-hydroxy-4-phenylselenotetrahydropyran and its (2S,3S, 4S) diastereomer. Elimination of the selenoxide, derived from oxidation of the former with hydrogen...
Altogether, this dissertation highlights the value of mass spectrometry-based analysis of intact proteins for solving problems related to understanding disease mechanisms and developing new therapeutics.
Mutations to the antioxidant enzyme Cu, Zn Superoxide Dismutase (SOD1) perturb protein structure in vivo to cause 2-5% of amyotrophic lateral sclerosis (ALS) cases. Metal-deficient...
Lycopodium alkaloids have shown widespread and noteworthy biological activity, consisting of over 250 known natural products. Moreover, their structural complexity and diversity have attracted considerable attention from numerous laboratories including our own. Recently, we have developed a unified approach that would provides access to numerous, previously unprepared C₁₀-functionalized lycopodium alkaloids....
This dissertation is separated into two parts according to the two major distinct research projects. In Part I, the full account of synthetic studies toward C10-functionalized lycopodium alkaloids is described. In Part II, the detailed discussion on the exploration of the Pummerer cyclization methodology and its application to the total...
Prostaglandin F₂α (PGF₂α), a stimulatory hormone of luteal oxytocin
secretion, is known to activate protein kinase C (PKC); however, the
intracellular signals that promote exocytosis of oxytocin remain to be
elucidated. Myristoylated alanine-rich C kinase substrate (MARCKS), a
protein specifically phosphorylated by PKC, crosslinks actin filaments
associated with the inner...