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  • Physico-chemical studies on the structure of eukaryotic chromatin

    Creator:
    Sahasrabuddhe, Chintaman Gopal
    Abstract:
    It has been shown that about 50 % of the whole chromatin DNA exists in a very compact form in calfthymus chromatin.These compact fragments can be extracted from the whole chromatin as nuclease resistant fragments by limited nuclease digestion. These fragments involve four out of five histones in folding the...
    Resource Type:
    Dissertation
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    EUKARYOTIC CHROMATIN Redacted for privacy AAbstractapproved: K.E.Van Hoede / It has been shown that about

  • The effects of various factors on the formation and fate of extranuclear chromatic bodies in Tetrahymena pyriformis

    Creator:
    Salamone, Michael
    Abstract:
    During karyokinesis in the ciliate, Tetrahymena pyriformis, strain GL-I, an extranuclear chromatin body (ECB) is frequently formed as an excluded mass between the two dividing daughter macronuclei. The formation and fate of ECB's were followed by means of Feu lgen, toluidine blue 0, and euchrysine staining and autoradiography (³H-thymidine). The...
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    Dissertation
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    EFFECTS OF VARIOUS FACTORS ON THE FORMATION AND FATE OF EXTRANUC LEAR CHROMATIN BODIES IN TETRAHYMENA

  • Chromatin structure : possible regulatory role in gene expression

    Creator:
    Herman, Timothy Merle
    Abstract:
    Chromatin DNA sequences intimately associated with the "core" of the basic, repeating chromatin subunit are shown to be relatively resistant to digestion by micrococcal nuclease. Chromatin DNA sequences lying between adjacent chromatin subunits, as well as DNA sequences in regions of chromatin which are free of chromatin subunits, are subject...
    Resource Type:
    Dissertation
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    Chromatin structure : possible regulatory role in gene

  • Physical studies of the yeast inner histones

    Creator:
    Mardian, James Kenneth Walker
    Abstract:
    In this work, the four yeast inner histones were prepared by the use of consecutive slab gel fractionation. The use of this technique allowed the first complete fractionation and rigorous identification of the four yeast inner histones, and made possible a study of their complexing behavior. Variants and adjuncts of...
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    Dissertation
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    His tones The Complexing Pattern of the Inner Histones and Chromatin Structure Preparative Gel

  • Physical properties of calf thymus H1 histones : conformational changes and interactions with two nonhistone chromosomal proteins

    Creator:
    Smerdon, Michael John
    Abstract:
    This work represents the first study of the conformational changes in histone H1 subfractions as well as the interactions of these subfractions with two nonhistone chromosomal (NHC) proteins. Calf thymus Hi was fractionated by the method of Kincade and Cole (1966a) using a very shallow guanidinium chloride gradient. A possible...
    Resource Type:
    Dissertation
    Full Text:
    chromatin. Studies on the composition of chromatin show a DNA:protein:RNA ratio of approximately 1.0:2.5

  • Physical structure and reconstitution of chromatin core particles

    Creator:
    Tatchell, Kelly George
    Abstract:
    The nucleosome appears to be the basic structural unit of the eukaryotic chromosome. The usual nucleosome preparation, isolated from a micrococcal nuclease digest of nuclei, is heterogenous in DNA length and protein composition with some particles containing spacer DNA and associated protein. This heterogeneity complicated physical and reconstitution studies. As...
    Resource Type:
    Dissertation
    Full Text:
    Physical structure and reconstitution of chromatin core

  • Structure and function of chromatin : studies at the nucleosome and nuclear matrix levels

    Creator:
    Shih, Ah Shing
    Abstract:
    Several levels of eukaryotic chromatin structure have been observed: the nucleosome, the 10 nm and 30 nm fibers and loop domains, apparently attached to the nuclear matrix. In this research, the structure and function of chromatin at two of these levels was investigated, with studies on both nucleosome positioning and...
    Resource Type:
    Dissertation
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    Structure and function of chromatin : studies at the

  • The binding of ethidium bromide to chromatin : model for carcinogen interactions

    Creator:
    McMurray, Cynthia T.
    Abstract:
    Five forms of chromatin, which represent the in vivo folded forms of nucleic acid, were isolated and used as the binding substrate for model intercalating compound, ethidium bromide. For all forms of chromatin, the affinity, location and structural effects of binding were examined. On the level of the nucleosome, the...
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    Dissertation
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    The binding of ethidium bromide to chromatin : model for

  • In vitro transcription in the yeast: Saccharomyces cerevisiae

    Creator:
    Ide, Gregory James
    Abstract:
    The structure and transcriptional activity of intranuclear and isolated chromatin from logarithmically growing yeast cells has been compared to chromatin from cells which have entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern and a 160 by repeat size when digested with staphlococcal nuclease....
    Resource Type:
    Dissertation
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    activity of intra- nuclear and isolated chromatin from logarithmically growing yeast cells has been

  • Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

    Creator:
    Dong, Feng
    Abstract:
    By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable...
    Resource Type:
    Dissertation
    Full Text:
    "tails" with respect to the structure, stability, and function of chromatin AN ABSTRACT OF THE THESIS