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  • RFLP analyses of interspecific hybrids of Phaseolus

    Creator:
    Kuo, Mei
    Abstract:
    Gene transfer via interspecific hybridization within Phaseolus has been limited by abnormal hybrid development and the rapid loss of hybridity in subsequent generations. The most advanced embryo development occurs in P. vulgaris x P. coccineus where mature seeds form. However, the reciprocal cross still results in abnormal embryos unable to...
    Resource Type:
    Dissertation
    Full Text:
    Interspecific Hybrids of Phaseolus Abstract approved: ^ David W.S. Mok (Professor) Gene transfer via

  • Characterization of cytokinin metabolic enzymes from Phaseolus

    Creator:
    Dixon, Susan C.
    Abstract:
    An enzyme catalyzing the formation of O-glucosylzeatin in immature embryos of P. lunatus was purified 2500 fold using ammonium sulfate precipitation followed by affinity and anion exchange chromatography. The enzyme uses trans-zeatin as substrate (Km 28 uM) but not cis-zeatin. ribosylzeatin, or dihydrozeatin. Both UDP-glucose and UDP-xylose can serve as...
    Resource Type:
    Dissertation
    Full Text:
    Metabolic Enzymes from Phaseolus Abstract approved: ^„ ^ r;~. ,, ^ - . Dr. David W. S. Mok

  • Zeatin O-xylosyltransferase in Phaseolus vulgaris : immunological and molecular analyses

    Creator:
    Martin, Ruth C.
    Abstract:
    Cytokinins are a group of plant hormones mediating cell division and differentiation. Zeatin is a naturally occurring and highly active cytokinin that is rapidly metabolized in Phaseolus seeds. The metabolites and enzymes mediating the conversion have been studied extensively. Zeatin metabolic enzymes may be utilized in studying the regulation of...
    Resource Type:
    Dissertation
    Full Text:
    Phaseolus vulgaris: Immunological and Molecular Analyses Abstract approved: , ^6avid W. S. Mok

  • Developmental mutants of Phaseolus vulgaris L.

    Creator:
    Shii, Chou Tou
    Abstract:
    Hybrids with abnormal development were obtained when particular genotypes of Phaseolus vulgaris L. were crossed. The phenotypic aberrations of the F₁s included retarded growth, chlorosis of the trifoliate leaves and the formation of adventitious roots on the hypocotyls. The expression of the abnormalities was dependent on the temperature. At high...
    Resource Type:
    Dissertation
    Full Text:
    Phaseolus vulgaris L. Abstract approved: __ Dr. David W. S. Mok Hybrids with abnormal

  • Embryo development in relation to interspecific hybridization of Phaseolus

    Creator:
    Rabakoarihanta, Aimee
    Abstract:
    Interspecific hybridizations between P. vulgaris and P. acutifolius and between P. vulgaris and P. lunatus were investigated. Prefertilization events were similar in inter- and intra-specific crosses. Fertilization was completed in all crosses and the time of fertilization was dependent on the maternal parent, reflecting differences in the time of maturation...
    Resource Type:
    Dissertation
    Full Text:
    / - ^ - - r ^ Dr. David W. S. Mok Interspecific hybridizations between P_. vulgaris and P_