A sucrose density-gradient ultracentrifugation technique for
obtaining microsomes has been developed. This technique has
advantages over the classic differential centrifugation technique, in
that it is faster, is better suited to work with small quantities of
tissue, and offers the opportunity to work with a defined microsomal
sub-cellular fraction, rather than...
The induction of drug-metabolizing enzymes is a
complex biochemical process manifested not only by
increases in various cytochrome P-450 isozymes and
related activities, but also by alterations in
endoplasmic reticulum membranes. Little work has been
done to examine how cytochrome P-450 inducing agents
influence other cellular processes, such as
phospholipid...
Cotoneaster is an ornamental shrub valued for showy flowers, berries and architecture as well as the ability tolerate adverse conditions under which other taxa fail. Cotoneaster is a highly diverse genus of over 400 species, of which few are available in the US nursery trade. Some species commercially available have...
The benefits of reproduction are clear, but there are also costs. Much is known about the costs of reproduction in females, but only recently have male costs been investigated in any depth. These costs of reproduction may be minimized by appropriately modifying behavior, but there has been little research on...
The common cherrylaurel (Prunus laurocerasus) and Portuguese cherrylaurel (P. lusitanica) are economically important landscape shrubs across the United States. To gain a better understanding of physiology and morphology in these higher level polyploids (P. lusitanica, 2n = 8x; P. laurocerasus, 2n =22x), a variety of breeding experiments were performed. The...
Buddleja davidii (butterfly bush) is a highly adaptable landscape plant known for pollinator attraction. Unfortunately, it is also regarded as an invasive plant in highly disturbed environments across the globe. It is an invasive species in Oregon, officially classified as a class B noxious weed in 2004. Currently the state...
Landscape plants are highly diverse and nursery producers often grow thousands of taxa to satisfy the varied needs and aesthetic tastes of consumers. Related to this diversity, ornamental plant breeders must be nimble and diverse in their approach. Two seemingly disparate studies were conducted to investigate improving ornamental shrubs –...
We report the use of MIL-120 as a water-stable and cost-effective metal-organic framework (MOF) for selectively capturing CO₂ from wet flue gas. Synthesized using inexpensive and environmentally benign reagents in water, MIL-120 possesses one-dimensional pores decorated with hydroxyl-bridged Al(III) ions and benzene rings with an interstitial spacing of 4.78 Å....
High Entropy Alloys (HEA) exhibit unusual properties and are gaining interest as new materials for challenging applications in the aerospace, hypersonic and medical fields. The application of HEAs have been limited by the development of powder production at scale. A recently developed process of plasma spheroidization by 6K, inc. has...
Post-tensioned (PT) self-centering shear walls were designed to reduce structural damage from seismic activity. However, these lateral force resistant systems experience post-tension loss negatively affecting the re-centering capability due to delayed deformations experienced by engineered wood products over time. The long-term mechanical response of mass-timber panels to in-plane PT loads...
The extent and severity of wildfires in forested regions are increasing throughout many regions on the planet, including western North America. High-severity wildfires directly affect soils and vegetation by altering soil hydraulic properties, reducing soil organic matter, exporting carbon and nitrogen, and killing trees and understory vegetation. These impacts can...
Hereditary variation is a vital component in the development of new and improved cultivars of landscape plants. Sources of hereditary variation include naturally occurring variation, recombination due to controlled crosses, artificial mutagenesis, and genetic modification via biotechnology. Here I explore all methods with the exception of genetic modification via biotechnology....
Oregon State University campus grounds have long been admired for their beauty and the diversity of over 65,000 individual woody plants that grace the 570 acres of campus. The woody plant collection has provided a living learning laboratory for the university and surrounding community for decades. The campus grounds include...
Butane-grown cells of Pseudomonas butanovora, Nocardioides sp. CF8 and Mycobacterium vaccae JOB5 were tested for their ability to cooxidize methane, ammonia and ethylene. Less than 10 nmol of methane were degraded by each of the bacteria (0.17-0.35 mg protein) in 30 minutes. Hydroxylamine and nitrite accumulated when Nocardioides CF8 and...
Hemp is a dioecious, annual, flowering plant in the Cannabaceae family. It has been used for thousands of years to produce textiles, essential oils, and medicines. The overall purpose of these experiments was to propagate the cannabinoid-free cultivar, ‘I3’, and follow up by inducing polyploidy in the ‘I3’ hemp via...
The black vine weevil (BVW), Otiorhynchus sulcatus (F.) (Coleoptera:
Curculionidae) is a persistent pest of nursery operations in the United States,
feeding on over 140 species of plants. The goals of this research were to 1) assess
the behavioral response of BVW to a commonly employed insecticide bifenthrin
(Talstar 0.2G®)...
A literature review and a computer study are conducted to identify
the requirements of finite element programs for the specific use of discrete
analyses of reinforced soil structures. The utility of six commercially
available programs are appraised with respect to the identified
requirements. The results of the study are directed...
In the U.S., the Nuclear Regulatory Commission (NRC) is responsible for setting annual dose limits. For cases of hot particle contamination these limits are set at depths in skin of 7 mg/cm², 300 mg/cm², and 1000 mg/cm². However, at such shallow depths, the lack of charged particle equilibrium (CPE) precludes...
In the area that is now Saguaro National Park East, grazing began in the 1880's. Because of the impact to the iconic saguaro cactus as well as all palatable plant species in the area, ecological damage in the park were determined to be so great that anti-grazing conservationists challenged the...
Successful cryopreservation of bone marrow in situ within vertebral bodies offers an opportunity for quick and convenient storage of a significant quantity of bone marrow stem cells, including hematopoietic- and mesenchymal-type stem cells. However, cryopreservation of more complex large tissues and organs, such as the vertebral body (VB), remains problematic....
The European hazelnut (Corylus avellana L.) is a diploid (2n = 2x = 22) tree crop important to the economy of Oregon’s Willamette Valley, where 99% of hazelnut production in the United States is located. Corylus avellana exhibits sporophytic self-incompatibility (SSI), controlled by a single S-locus with at least 33...
In watersheds across the American Pacific Northwest, changes in the cultural and regulatory landscape have increased pressure to restore and protect populations of anadromous fish. But restoration of anadromous fish populations constitutes a ‘wicked problem’, relentless in character, affecting diverse stakeholder groups, and defying ‘once and for all’ solutions (Weber...
The nearshore waters off the Oregon coast (< 73 meters) are a region of high productivity and economic value, with a variety of habitats that include rock outcrops. Temperate reef habitats are important to many commercially important fishes inhabiting the Pacific coast, including canary rockfish (Sebastes pinniger) and yelloweye rockfish...
Transformation is a major bottleneck for genetic engineering and gene editing in forest tree species. This includes most genotypes of Populus and Eucalyptus, which are some of the world’s most widely-cultivated genera of plantation forest trees. To provide new tools for transformation, I tested the transcription factor-protein chimera consisting of...
Semiconducting materials which can be ambipolarly doped are highly desirable in many electronics applications, including use as solar cell materials. SnZrS₃ is being investigated for the possibility of ambipolar doping, with potential applications as a solar cell absorber layer. This dissertation covers the synthesis of SnZrS₃ and the related compound...
Ankle injuries comprise more than 15% of all sports injuries worldwide. The efficacy of the ankle taping for injury prevention has long been under scrutiny as numerous studies have shown that tape rapidly loses its ability to constrain ankle motion with exercise. Consequently, ankle braces (orthoses) are being used with...
Although the concept of breeding crops for organic agricultural systems has been around for decades, few studies have been reported for vegetable crops to quantify and compare how conventional and organic systems differ in their selection pressures on genotypes advancing through the breeding cycle; especially research utilizing modern sequencing capabilities....
Highbush blueberry (Vaccinium corymbosum L.) is adapted to soils with high organic matter and acidic pH. Composts provide organic matter and nutrients for growing blueberry in mineral soils, but many composts are high in pH and soluble salts. Acidification with elemental sulfur (S°) can remedy high pH, but the process...
The standard way of representing numbers on computers gives rise to errors
which increase as computations progress. Using p-adic valuations can reduce
error accumulation. Valuation theory tells us that p-adic and standard valuations
cannot be directly compared. The p-adic valuation can, however, be used in
an indirect way. This gives...
Nanoparticles have become more prevalent from their use in sunscreen to antimicrobial agents in socks. In light of these applications, gaining a fundamental understanding of how NPs interact with humans is crucial for their future. Specifically, how cells interact with NPs and what factors drive the modes of cellular uptake....
A discrete multi-facility plant location algorithm developed by Basheer Khumawala is modified and applied to the location analysis of offices of the State of Oregon Health Facilities Licensing and Certification Section (HFLC). Choice of the algorithm is based upon a literature survey of available computational procedures which compares their relative...
Krascheninnikovia lanata (winterfat) is a valuable forage species with an
average of 10% crude protein during winter when there are few nutritious options for
livestock and wildlife. However, excessive grazing throughout the west has negatively
impacted survival of winterfat stands. We hypothesized that four years rest from
dormant season livestock...
Relative to visual and audible communication, haptic perception is quiet, does not require eye contact, can be applied at any point on the human body, and can function in parallel with the other four human senses. Vibrotactile feedback is a method of haptic communication using vibrations applied to the skin....
The purpose of this study is to investigate the relative performance of compact ionization chambers as it changes based on the speed of detector motion and collection volume. To quantify changes, multiple scans were made with each of a selection of compact chambers and repeated varying detector speed. Each scan...
Statistical data from breeding experiments suggested that a
reciprocal chromosomal translocation had spontaneously occurred in
a line of S. C. White Leghorn fowl. Approximately 50% of the
embryos from matings between suspected translocation heterozygotes
and normal birds died early during incubation. In an effort
to obtain cytological proof of this...
Dietary cyclopropenoid fatty acids (CPFA) have long
been known to cause pronounced physiological disorders in
both farm and laboratory animals. Past work has shown
CPFA to be a powerful promoter of carcinogenesis in trout,
rats and mice. Sterculic and malvalic acids (CPFA's) are
found in seed lipids of plants from...
The performance of a combined vapor-compression cycle/ORC is evaluated using waste-heat from a diesel generator. A flat plate microchannel heat exchanger is employed to provide energy exchange between the diesel exhaust stream and an oil loop, which provides energy to a boiler. This study finds an increased diesel duty corresponds...
Unmanned aerial vehicle (UAV) technology has grown out of traditional research and military applications and has captivated the commercial and consumer markets, showing the ability to perform a spectrum of autonomous functions. This technology has the capability of saving lives in search and rescue, fighting wildfires in environmental monitoring, and...
A method for improving performance/watt of an embedded single-instruction multiple-data (SIMD) architecture using application-guided a priori scheduling of hardware resources is presented. A multi-core architectural simulator is adopted that accurately estimates power, performance, and utilization of various processor components (logic, interconnect and memory). A greedy search is then performed on...
The effect of raised bed culture on winter survival of ten pea
genotypes (Pisum sativum L.), differing in inherent cold hardiness
and growth habit, was studied under two climatic conditions: a moist
semi-maritime winter (Corvallis, Oregon) and a cold continental
winter (Weston, Oregon). An assessment of winter injury was based...
A gas-permeable-membrane-supported (GPMS) biofilm consisting
of methylotrophic bacteria was effective in degrading chlorinated
methanes, ethanes, and ethenes. The biofilm was developed on a
gas-permeable fabric (Goretex, W.L.Gore & Associates, Elkton,
Maryland) that divided a reactor vessel into a liquid compartment
and a gas compartment. Goretex is a nylon-backed teflon mesh...
Graphene and its derivatives are two-dimensional hexagonal lattices that possess desirable physicochemical properties leading to an ever-expanding list of industrial and biomedical uses. Graphene oxides (GOs) are becoming a popular graphene alternative and while there have been human safety studies, investigations of developmental toxicity are lacking. The use of distinct...
Traditionally sinusoidal signal generation has been implemented with purely analog circuits such as phase-locked loops. The alternative of using a digital system to perform this signal generation has previously been unattractive due to limitations in clock frequency and size. However, recent advancements in sub-micron fabrication techniques have made the digital...
The movement of Chinook salmon through space and time, across political boundaries, and through fisheries, creates one of the most complex marine resource management problems in the world. Information garnered from the recovery of coded-wire tags (CWTs) has been used since the 1970s to direct management decisions. Growing concern surrounding...
Steel foundries are in great need of automation as current operations involve many hazardous manual tasks. Automating foundry operations is very challenging due to the variety of tasks that must be performed on physical objects that vary significantly in size, shape, and weight. This thesis focuses on robotically automating the...