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Growth properties of induced mutants of the common wheat-bunt fungus, Tilletia caries, in axenic culture and in planta

Creator:: Churchill, Alice Cynthia Louise
Abstract:: Genetic studies of Tilletia spp. have been hampered by the lack of genetic markers. The work discussed here is a first report of the isolation of genetically marked strains of J. caries and their use in wheat plant infection studies. Procedures for the isolation of auxotrophic and drug-resistant mutants in...
Resource Type:: Masters Thesis
Full Text:: AXENIC CULTURE AND IN PLANTA Abstract approved: Redacted for Privacy Darrice I. Milfs Genetic studies

Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola

Creator:: Quant, Rebecca L.
Abstract:: Five strains of the bean pathogen Pseudomonas syringae pv. phaseolicola were analyzed for plasmid number, size, and relatedness. Each strain contained one or two indigenous plasmids which ranged in size from 6.8 to 151 kbp. BamHI and EcoRI restriction patterns revealed that total plasmid DNA from each strain had a...
Resource Type:: Masters Thesis
Full Text:: Dallice I. Mills Five strains of the bean pathogen Pseudomonas syringae pv. phaseolicola were analyzed

Genetic recombination of the integrative plasmid pMC7105 of Pseudomonas syringae pv. phaseolicola involves repetitive sequences

Creator:: Szabo, Leslie J.
Abstract:: A strain of the phytopathogenic bacterium Pseudomonas syringae pv. phaseolicola, a pathogen of common bean Phaseolus vulgaris, harbors a 150 kilobase pair (kb) plasmid pMC7105. In one strain, LR719, pMC7105 is stably integrated in the bacterial chromosome. Imprecise excision of the pMC7105 replicon has been shown to result in the...
Resource Type:: Dissertation
Full Text:: . phaseolicola Involves Repetitive Sequences Abstract Approved: Redacted for privacy Dallice I. Mills A strain

Integration and excision of pMMC7105 in Pseudomonas syringae pv. phaseolicola occur by recombination between copies of a repetitive IS-like sequence

Creator:: Ehrenshaft, Marilyn
Resource Type:: Dissertation
Full Text:: Approved: Redacted for Privacy Dallice I. Mills The chromosomal and plasmid sites for integration of

The growth of wild-type and genetically-marked strains of Tilletia caries and T. controversa in susceptible and resistant wheat cultivars

Creator:: Trail, Frances
Abstract:: The objective of this study was to provide basic information on the interaction of the bunt fungi Tilletia caries and T. controversa with their host, Triticum aestivum. Studies on the ability of haploid strains to colonize wheat heads provided the basis for experiments in which haploid strains were crossed in...
Resource Type:: Masters Thesis
Full Text:: ACKNOWLEDGEMENTS I would like to thank Dr. Dallice Mills for his support, encouragement and enthusiasm during the

Characterization of the replication, incompatibility, and recombination regions of Pseudomonas syringae pv. phaseolicola plasmid pEXC8080

Creator:: Poplawsky, Alan R.
Resource Type:: Dissertation
Full Text:: : Dallice I. Mills Pseudomonas syringae pv. phaseolicola is a pathogen of common bean Phaseolus vulgaris

Electrophoretic karyotypes and molecular genetic analysis of Tilletia caries and T. controversa

Creator:: Russell, Brian W.
Abstract:: Electrophoretic karyotypes were obtained from intact cells of four wild type strains of Tilletia caries and T. controversa, and 5 inter specific hybrid progeny using clamped homogeneous electric field, pulsed field gel electrophoresis (CHEF PFGE). Each karyotype was polymorphic and unique relative to the other strains. However, the relative size...
Resource Type:: Masters Thesis
Full Text:: : Dal lice I. Mills Electrophoretic karyotypes were obtained from intact cells of four wild type

Molecular and genetic analyses of genome variability in Ustilago hordei

Creator:: Agnan, Jacqueline
Abstract:: Pulsed-field gel electrophoresis (PFGE) of the strains representing the 14 races of U. hordei, the causal agent of covered smut on barley, revealed extensive polymorphism in chromosome length and number. The purpose of this study was to determine by two approaches the exact chromosome number for each U. hordei race,...
Resource Type:: Dissertation
Full Text:: of genome variability in Ustilago hordei. Abstract approved. Dal lice I. Mills Pulsed-field

Preliminary biochemical evidence for an ascospore coat protein in Saccharomyces cerevisiae : partial characterization and timing of synthesis

Creator:: Honda, Sandra
Abstract:: Sporulation in the yeast Saccharomyces cerevisiae involves the process of meiosis which is accompanied by ascospore formation. Using intact cells or spores, preliminary biochemical evidence is given to identify ascospore wall components not found in vegetative cell walls. Cell surface components are solubilized by treatment with 8 M urea and...
Resource Type:: Masters Thesis
Full Text:: Page I. INTRODUCTION 1 A. Overview of the Problem 1 B. Ascospore Formation 2 1. Physiology and

Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads

Creator:: Curiale, Michael Steven
Abstract:: Six plant pathogenic isolates of Pseudomonas syringae pv. glycinea, the causal agent of bacterial blight of Glycine max (L.) Merrill, and one isolate of P. syringae pv. phaseolicola, the causal agent of halo blight of Phaseolus vulgaris L., have been examined for the presence of circular duplex deoxyribonucleic acid (DNA)....
Resource Type:: Dissertation
Full Text:: Curiale ACKNOWLEDGEMENT I wish to express my deep appreciation to Dr. Dallice Mills, my major

ScholarsArchive@OSU

Growth properties of induced mutants of the common wheat-bunt fungus, Tilletia caries, in axenic culture and in planta

Molecular relationships among multiple-sized plasmids of Pseudomonas syringae pv. phaseolicola

Genetic recombination of the integrative plasmid pMC7105 of Pseudomonas syringae pv. phaseolicola involves repetitive sequences

Integration and excision of pMMC7105 in Pseudomonas syringae pv. phaseolicola occur by recombination between copies of a repetitive IS-like sequence

The growth of wild-type and genetically-marked strains of Tilletia caries and T. controversa in susceptible and resistant wheat cultivars

Characterization of the replication, incompatibility, and recombination regions of Pseudomonas syringae pv. phaseolicola plasmid pEXC8080

Electrophoretic karyotypes and molecular genetic analysis of Tilletia caries and T. controversa

Molecular and genetic analyses of genome variability in Ustilago hordei

Preliminary biochemical evidence for an ascospore coat protein in Saccharomyces cerevisiae : partial characterization and timing of synthesis

Physical properties and genetic studies of cryptic plasmids discovered in plant pathogenic pseudomonads

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