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  • Metabolic engineering of cyanobacteria to optimize photobiological hydrogen production

    Creator:
    Luterra, Markael
    Abstract:
    Photobiological hydrogen production -- the use of photosynthesis to split water into hydrogen and oxygen -- presents an opportunity for efficient and large-scale solar biofuel development. One of the major challenges in this area is competition for reductant between the hydrogen-evolving hydrogenase enzyme and other metabolic pathways. In this work...
    Resource Type:
    Dissertation
    Full Text:
    Metabolic Engineering of Cyanobacteria to Optimize

  • Advances in expression of heterologous protein products by Streptococcus gordonii

    Creator:
    Warren, Travis Kent
    Abstract:
    Streptococcus gordonii is a bacterial species that naturally colonizes the oral cavity of most healthy humans. It resides in the mouth as an adherent to dental surfaces and, with few exceptions, does not cause disease in individuals it inhabits. It possesses qualities that encourage its use as a vector to...
    Resource Type:
    Dissertation
    Full Text:
    growth medium enhanced the efficiency with which S. gordonii assimilated genetic material, thereby

  • Stability of reporter gene expression and RNAi in transgenic poplars over multiple years in the field under vegetative propagation

    Creator:
    Li, Jingyi
    Abstract:
    Stable expression of transgenes is required for commercial uses of genetically engineered trees. To better understand the stability of transgene expression under field conditions, we studied transgene expression and RNA interference (RNAi)-induced transgene suppression in 2,480 transgenic poplars (460 transgene insertion events) over three years. Stability of expression was assessed...
    Resource Type:
    Dissertation
    Full Text:
    Genetic engineering (GE), the use of recombinant DNA and asexual gene transfer methods to alter the

  • Exploration of the feasibility of developing a winter hardy 'Marion' blackberry through genetic engineering

    Creator:
    Meng, Rengong
    Abstract:
    Experiments focusing on plant growth regulators’ concentrations and combination, mineral salt formulations and TDZ pretreatment formations were conducted to optimize in vitro shoot regeneration from leaf and petiole explants of 'Marion' blackberry. Optimum shoot formation was obtained when stock plants were incubated in TDZ pretreatment medium for three weeks before...
    Resource Type:
    Dissertation
    Full Text:
    ' BLACKBERRY THROUGH GENETIC ENGINEERING RENGONG MENG 2 AN ABSTRACT OF THE

  • From the Oregon Wolfe Barley to fall-sown food barley : markers, maps, marker-assisted selection and quantitative trait loci

    Creator:
    Chutimanitsakun, Yada
    Abstract:
    Understanding complex traits is a fundamental challenge in plant genetics and a prerequisite for molecular breeding. Tools for trait dissection are markers, maps, and quantitative trait locus (QTL) analysis. Marker-assisted selection (MAS) is an application that integrates these tools. In this thesis research, a new sequence-based marker was evaluated, maps...
    Resource Type:
    Dissertation
    Full Text:
    walls of starchy endosperm and aleurone cells. The genetic basis of β-glucan is remarkably complex and

  • Identifying the Parameters for Wheat Pollen Electroporation Transformation

    Creator:
    Liu, Jiang
    Abstract:
    Wheat (Triticum aestivum L.) is one of the most important crops in the world. It has been and continues to be one of the main sources of food for humans and animals. Although traditional wheat breeding has contributed greatly to the improvement of wheat both in productivity and biotic/abiotic stress...
    Resource Type:
    Masters Thesis
    Full Text:
    .     Bhalla,  P.  L.  (2006).  Genetic  engineering  of  wheat–current  challenges  and

  • Biological considerations for a new approach to regulating genetically modified crops in the United States

    Creator:
    McDonough, Ashley
    Abstract:
    The debate over GM crops is one of the most controversial issues of recent decades. The debate has many dimensions, including environmental impacts, benefits and risks to human health, intellectual property rights, adequacy of biosafety regulations, and fundamental ethical acceptability. The potential benefits of GM crops are numerous, including improved...
    Resource Type:
    Honors College Thesis
    Full Text:
    crops in the United States Introduction to the Issue of Genetically Modified Crops Genetic engineering

  • Advances in Freshwater Cyanobacterial Genomics

    Creator:
    Brown, Nathaniel M.
    Abstract:
    Limnology is undergoing a transition to high-throughput -omic analysis of freshwater bacterial communities. An important first step in making the transition is to characterize several genomes that can be used as references to guide metagenome assembly and analysis. Here I characterize four new freshwater cyanobacterial genomes, a pair of lake...
    Resource Type:
    Dissertation
    Full Text:
    Cyanobacteria . . . . . . . . . . . . 104 5.3.2 Isolation of a novel freshwater temperate Siphovirus, C-CRS01105

  • Silencing the Agrobacterium tumefaciens oncogenes

    Creator:
    Pitrak, Jennifer
    Abstract:
    Crown gall disease is an agricultural problem caused by the soil-borne bacterium, Agrobacterium tumefaciens. A. tumefaciens oncogenes cause transformed plant cells to overproduce the hormones, auxin and cytokinin. High hormone levels cause unorganized plant cell growth resulting in a gall. Control of crown gall disease is difficult because after plant...
    Resource Type:
    Dissertation
    Full Text:
    genetic overlap among the different RNA silencing pathways (Figure 1.6) (Table 1.2). Table 1.2

  • Construction of a model organism for performing calcium carbonate precipitation in a porous media reactor

    Creator:
    Kaufman, Megan J.
    Abstract:
    Aquifers are an important storage location and source of fresh groundwater. They may become polluted by a number of contaminants including mobile divalent radionuclides such as strontium-90 which is a byproduct of uranium fission. A method for remediating such divalent radionuclides is sequestration through co-precipitation into calcium carbonate. Calcium carbonate...
    Resource Type:
    Masters Thesis
    Full Text:
    much research has gone into engineering biofilm barriers. Biofilm barriers can be used in