Proteins are the metabolic machines of the cell and as such, the study of proteins could illuminate the dominant biological activities that are occurring within cells and reveal how an organism interacts with its environment. Here, we used proteomic techniques to study the abundant marine bacterium SAR11 both as an...
The objective of this study was to examine the variation among ammonia-oxidizing and denitrifying communities in soils of a natural Fennoscandian boreal forest and of a forest with a long history of fertilization with different levels of nitrogen (N), and to examine whether there are any changes in the microbial...
Prior to 2005, ammonia oxidation, the first step of nitrification, was thought mediated mainly by ammonia-oxidizing bacteria (AOB). However, the discovery of Thaumarchaeota carrying the genes coding for the ammonia monooxygenase (AMO) enzyme led to the discovery that ammonia-oxidizing archaea (AOA) also contribute to nitrification. Despite the uptick in studies...
The rising demand on freshwater resources emphasizes the benefit of biogeochemical processes that maintain clean water by removing pollutants such as excess nitrogen. The most sensitive step in the removal of nitrogen from waterways is the oxidation of ammonia, a part of nitrification, which is carried out by ammonia oxidizing...
Identifying the inhibition of ammonia oxidizing bacteria (AOB) by emerging organic contaminants is crucial due to the importance of AOB in wastewater treatment, the widespread use of antibacterial agents such as triclosan (TCS) in consumer products, and the sensitivity of N. europaea to inhibitors. Triclosan inhibition of nitrification by AOB...
In most environments, ammonia (NH₃) is oxidized to nitrate (NO₃⁻) via the intermediate nitrite (NO₂⁻). This is a microbe-driven process involving phylogenetically distinct types of microorganisms, namely, ammonia-oxidizing bacteria (AOB) and Thaumarchaea (AOA), and nitrite-oxidizing bacteria (NOB). Nitrosomonas europaea and Nitrobacter winogradskyi are the best studied AOB and NOB, respectively,...
Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
The inhibitory effects of Corexit 9500A, Alaska North Slope Crude oil (ANSC), and mixtures of the two on the ammonia-oxidizing bacteria, Nitrosomonas europaea, and the ammonia-oxidizing archaea, Nitrosopumilus maritimus, were investigated. Corexit 9500A was found to be minimally toxic to both microorganisms with concentrations of 2000 and 3000 ppm yielding...
Historically, the difficulty of obtaining pure cultures of abundant marine
microbial plankton has an obstacle to reconstructing the underlying
mechanisms of biogeochemistry in the ocean. While a number of dominant
marine species from the ocean surface have been cultured, the dominant
microbial plankton of the dark ocean proved far more...
Butane-grown cells of Pseudomonas butanovora, Nocardioides sp. CF8 and Mycobacterium vaccae JOB5 were tested for their ability to cooxidize methane, ammonia and ethylene. Less than 10 nmol of methane were degraded by each of the bacteria (0.17-0.35 mg protein) in 30 minutes. Hydroxylamine and nitrite accumulated when Nocardioides CF8 and...