Replication of plasmid molecules containing either a left-end or a
right-end adenovirus origin was investigated. When the plasmids were
linearized to expose their respective adenovirus termini, mixed, and
transfected with helper adenovirus DNA into the human 293 cell line, a
new molecule was detected. The new molecule was the size...
The work in this thesis has provided conclusive genetic evidence
that "panhandle" intermediates form during adenovirus replication.
Adenovirus chromosomes lacking 51 by from their left -hand termini
are infectious and capable of regenerating the missing origin
sequence. Yet if an entire inverted terminal repeat is removed, the
adenovirus chromosome is...