Selected parameters that affect crude toxin production in both
primary culture (growing cell culture) and resting culture were
examined during efforts to produce sufficient crude toxin for purification.
The parameters included carbohydrate and label source and
concentration, cell concentration, incubation time and temperature
and shaker speed. A higher yield of...
Aflatoxins B₁, Q₁ and aflatoxicol, R [subscript o] F, but not B₂ were activated in the presence of NADPH by the 105,000 x g pellet from rainbow
trout (Salmo gairdneri), Mt. Shasta strain, liver to products lethal to
Bacillus subtilis GSY 1057 (metB4, hisAl, uvr-1). Electron microscopy
confirmed the microsomal...