The objective of this project was to investigate the effects of refrigeration storage and processing technologies on the quality and nutraceutical benefit of blackberries. 'Marion' and 'Evergreen', the two major blackberry varieties in Oregon, were evaluated in this study. For refrigeration storage, fresh fruit were packed in clam-shell containers right...
Blackberry (Rubus sp.) fruit are a good source of phenolic compounds and anthocyanins, and are consumed in fresh and processed forms. Though organic products become more popular, limited information is available about how different organic production methods affect the post-harvest quality of blackberry fruit. The objective of this project was...
Blackberry pomace (BBP), a byproduct from juice processing, is often disposed of in landfills. We propose BBP as an antioxidant-rich feed supplement for transition dairy cows. To explore the effects of BBP on antioxidant status, 24 multiparous dairy cows (5 Holstein and 19 Jersey) were assigned randomly to one of...
Shrimp waste samples were extracted with a variety of organic solvents. Each fraction was measured for antioxidant activity by determining the rate of oxidation of β-carotene-linoleic acid in an emulsion system. An ethanol extract exhibited the highest antioxidant activity. Purification of the most active fraction was accomplished by thin layer...
The effect of increased manganese superoxide dismutase (Mn-SOD) and cytosolic ascorbate peroxidase (cAPX) on various abiotic stresses was studied using transgenic tomato (Lycopersicon esculentum) plants. Transformants were selected in vitro based on kanamycin resistance, confirmed by PCR and northern blot analyses. Northern blots showed an enhanced gene expression of Mn-SOD...
Wine grape pomace (WGP), the byproduct from winemaking, is a good source of polyphenols and dietary fibers, and may be utilized as antioxidant dietary fibers (ADF) for food applications. The objectives of this thesis research were to first determine the phenolic compounds, antioxidant and antimicrobial activities in red WGP under...
The influence of cultivar and maturity on polyphenolic composition and
antioxidant activity was investigated by UV-Visible spectrophotometry and
analytical high performance liquid chromatography (HPLC) in commercially
important blackberry cultivars and selections. The anthocyanin pigments changed
tremendously with ripening. Total anthocyanins increased from 74.7-317 mg/100g
fw from underripe to over ripe...
The distribution of anthocyanin pigments and polyphenolics of sweet (Prunus avium) and sour cherries (Prunus cerasus) were determined by Ultraviolet- Visible (UV-Visible) spectrophotometry and High Performance Liquid Chromatography with photodiode array detector (HPLC-DAD). Their antioxidant properties were determined by Oxygen Radical Absorbance Capacity (ORAC) and Ferric Reducing Antioxidant Power (FRAP)....
Purple tomatoes (Lycopersicon esculemtum) were bred by incorporating
genes that result in anthocyanin production in the skin of fruits. These genes
originally came from related wild species as no naturally occurring genes encoding
fruit anthocyanin expression are known within the cultivated species. By
combining Aft or Abg with atv or...
Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...