The purpose of this investigation was to purify and characterize
the autolytic enzyme from cell walls of Bacillus subtilis 168. The
crude enzyme was obtained by autolysis of purified cell walls in
buffer at 37° C. Two purification methods were developed. The first
involved fractional precipitation with ammonium sulfate. The...
Hypersensitive response-like (HR-like) needle reactions to infection by the white pine blister rust pathogen, Cronartium ribicola, have been reported for several species of five needle pines native to western North America. The best-studied examples are in Pinus monticola and P. lambertiana. In these species a "needle spot" phenotype has been...