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Filtering by: Creator Utkhede, Raj S. Remove constraint Creator: Utkhede, Raj S. Subject Calpain -- Physiological effect Remove constraint Subject: Calpain -- Physiological effect

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  • Purification and characterization of recombinant calpain-5

    Creator:
    Wang, Mei-Chuan
    Abstract:
    Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....
    Resource Type:
    Dissertation
    Full Text:
    proteolytic activity. These results confirmed that calpain' s calcium-binding domain is important in

  • Effect of the calpain inhibitor E-64-d on the degradation of α-fodrin in damaged muscle

    Creator:
    Boyd, Jeffrey
    Abstract:
    We hypothesized that calpain activity is elevated in response to muscle damage. To test this hypothesis, we examined the degradation of α-fodrin into its 150 and 145 kDa fragments following either 20 eccentric or isometric contractions. In addition, experiments were performed in the presence or absence of E-64-d, a calpain...
    Resource Type:
    Masters Thesis
    Full Text:
    lengthening was 1.5 LF/s. The magnitude of stretch was calculated from the Lo measurement and a published LF