In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding
affinity for DNA than the isolated globular domain (GH5), supporting the importance of the...
The aim of this study was to gain more information about the
interactions between DNA and the histone octamer during the
process of transcription. This work used a pUC8 plasmid derivative
that contained the core promoter region of the RNA polymerase I of
Acanthamoeba castellanii, placed upstream of four repeats...
Several levels of eukaryotic chromatin structure have
been observed: the nucleosome, the 10 nm and 30 nm fibers
and loop domains, apparently attached to the nuclear
matrix. In this research, the structure and function of
chromatin at two of these levels was investigated, with
studies on both nucleosome positioning and...