Four experiments were conducted to determine oxytocin concen tration of bovine luteal tissue at different stages of the estrous cycle and to study the effects of prostaglandins F₂α (PGF₂α), E₂ (PGE₂), luteinizing hormone (LH), cycloheximide, colchicine and cytochalasin B on oxytocin synthesis and release in vitro. In experiment 1, luteal...
Two experiments were conducted to examine the effect of
a cyclopropenoid fatty acid on luteal cell function. In Exp.
1, 12 mature ewes were mated to a fertile ram, assigned to two
groups (n = 6/group) and laparotomized on day 18 of gestation.
Ewes with corpora lutea (CL) in both...
Two experiments were conducted to investigate the effects of
gonadotropin-releasing hormone (GnRH), administered twice during
the same estrous cycle, on bovine luteal function. Eight mature
beef cows, each cow serving as her own control, were injected
intravenously (i.v.) with saline on days 2 and 8 of the cycle (day of...
Experiments were conducted to examine the effects of a tumor-promoting phorbol ester, a calcium ionophore and indomethacin on bovine luteal oxytocin (OT) synthesis and secretion and progesterone secretion. Six 18-month-old crossbred beef heifers in Exp. 1 and five similar heifers in Exp. 2 were observed twice daily for estrus using...
To determine whether membrane-related events may be involved in
attenuated luteal function after gonadotropin-releasing hormone (GnRH)
administration, corpora lutea (CL) were removed from 10 beef heifers on day 7
of the estrous cycle after i.v. injection of GnRH or saline on day 2 of the cycle.
Luteal slices were incubated...
Interferon tau (IFN-τ) is the pregnancy recognition signal secreted by the trophectodermal cells of the developing bovine embryo that prevents luteolysis and maintains critical progesterone production. Recent evidence has suggested in addition to the mechanism of action identified in the uterus, there may be a direct effect of IFN-τ on...
Two experiments were conducted to evaluate the effects of
gonadotropin releasing hormone (GnRH) on the function of the bovine
corpus luteum during the estrous cycle. In Experiment 1, 10 heifers
were assigned randomly into two groups with each heifer serving as
her own control. Heifers in Group I (n=5) were...