The interaction of polylysine with DNA may be divided into two
separate phenomena: the primary interaction involved in formation of
a well-defined complex, and secondary interactions leading to aggregation
of these complexes. Most studies on the interaction of polylysine
with DNA have used techniques which measure properties of
the aggregated...
A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl
chloride (dansyl chloride), was covalently bonded to polylysine. The
dansyl tagged polylysine was complexed to DNA by gradient dialysis.
The polarization and intensity of fluorescence of the dansyl and the
turbidity of the complex were measured as a function of salt concentration
for a variety of...