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Filtering by: Creator Murray, Jessica R. Remove constraint Creator: Murray, Jessica R. Subject DNA Remove constraint Subject: DNA

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  • DNA precu[r]sor compartmentation in mammalian cells : metabolic and anti-metabolic studies of nuclear and mitochondrial DNA synthesis

    Creator:
    Bestwick, Richard Keith
    Abstract:
    This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
    Resource Type:
    Dissertation
    Full Text:
    of free bases and nucleosides. R N A DNA UTP CTP dCTP dTTP t4 CDP d CDP dU DP dCMP dUMP UdR

  • The association of DNA with other cellular materials in Escherichia coli lysates

    Creator:
    Porter, Bruce Wallace
    Abstract:
    The association of other materials with DNA has been studied extensively in higher organisms and more recently in bacteria. However, investigation of these complexes in bacteria have for the most part been restricted to the study of a specific function this association may serve, or a specific kind of association....
    Resource Type:
    Dissertation
    Full Text:
    concerned with the properties of DNP from calf thymus or other cells from higher organisms. Murray and

  • Materials associated with the DNA fraction of Bacillus subtilis W168

    Creator:
    Laudermilk, Patricia Joan
    Abstract:
    The purpose of this research was to determine if a basic protein was associated with the DNA of Bacillus subtilis W168, and to determine if there was a difference in the amount and type of protein associated with the DNA at different times during the growth cycle of the organism....
    Resource Type:
    Masters Thesis
    Full Text:
    for her valuable suggestions in the preparation of this thesis. To Dr. R. R. Becker for carrying

  • Effects of nucleosomes on transcription by polymerase I in a reconstituted system

    Creator:
    Georgel, Philippe, 1961-
    Abstract:
    The aim of this study was to gain more information about the interactions between DNA and the histone octamer during the process of transcription. This work used a pUC8 plasmid derivative that contained the core promoter region of the RNA polymerase I of Acanthamoeba castellanii, placed upstream of four repeats...
    Resource Type:
    Dissertation
    Full Text:
    150 mM NaC1 (R= 1.4 to R= 2.15). 4 2 2. 2. 2: Sedimentation velocity analysis of the reconstituted

  • Crystal structures of DNA four-way junctions

    Creator:
    Eichman, Brandt F.
    Abstract:
    DNA four-way junctions (also known as Holliday junctions) are the primary structural intermediate during recombination, an important process responsible for biological evolution and maintenance of genomes. These junctions arise from the assembly of four nucleic acid strands to produce double-helical regions extending from a central point. Although much progress has...
    Resource Type:
    Dissertation
    Full Text:
    ........................ 85 DEDICATION I dedicate this thesis to my wife Jessica. I am eternally grateful for her love

  • Site-specific aflatoxin B₁ adduction of sequence-positioned nucleosome core particles

    Creator:
    Moyer, Richard A.
    Abstract:
    The question of how the presence of nucleosomal packing of DNA modifies carcinogen interaction at specific sites cannot be answered by studies on whole chromatin or bulk nucleosomes because of the heterogeneity of DNA sequences in the particles. This problem was circumvented by constructing nucleosomes that are homogenous in DNA-histone...
    Resource Type:
    Dissertation
    Full Text:
    pronounced fluctuations in DMS binding. C T ) + ."T s I C r' . .'..r r! nt:V z.1911r7:;511-tr,7.;;T

  • Studies on the interaction of polylysine with deoxyribonucleic acid

    Creator:
    McKenzie, Raymond Leslie
    Abstract:
    The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
    Resource Type:
    Dissertation
    Full Text:
    developed by Huang, Bonner and Murray (32) to prepare soluble complexes of reconstituted DNP. In salt

  • The interaction of polylysine with DNA as studied by polarization of flurescence

    Creator:
    Evett, Jay Fredrick
    Abstract:
    A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride), was covalently bonded to polylysine. The dansyl tagged polylysine was complexed to DNA by gradient dialysis. The polarization and intensity of fluorescence of the dansyl and the turbidity of the complex were measured as a function of salt concentration for a variety of...
    Resource Type:
    Dissertation
    Full Text:
    Ti is the viscosity of the solution, R is the gas constant, T is the absolute temperature, and V is