Index Catalog // ScholarsArchive@OSU

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Creator:: Ji, Jiuping
Abstract:: A crucial factor in determining the accuracy of DNA replication is maintenance of a balanced supply of deoxyribonucleoside triphosphates (dNTPs) at replication forks. Perturbation of dNTP biosynthesis can induce dNTP pool imbalance with deleterious genetic consequences, including increased mutagenesis, recombination, chromosomal abnormalities and cell death. Using the T4 bacteriophage system,...
Resource Type:: Dissertation
Full Text:: Perturbations of DNA Precursor Biosynthesis in Phage T4 Redacted for Privacy Abstract Approved: Christopher K

Attempted cloning of T4 phage ribonucleotide reductase

Creator:: Booth, Joseph
Abstract:: Ribonucleotide reductase is an important enzyme in the control of DNA replication within the cell. Ribonucleotide reductase exerts its control through enzymatic reduction of nucleoside diphosphates. The bacteriophage T4 enzyme is an example of the class of iron-requiring reductases which also includes E. coli and mammalian ribonucleotide reductases. The two...
Resource Type:: Masters Thesis
Full Text:: . Clonability/Unclonability 24 J. Purpose of current work 25 MATERIALS AND METHODS 26 A. Enzymes for cloning 26

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Creator:: Martomo, Stella A.
Abstract:: Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Christopher K. Mathews Deoxyribonucleoside triphosphate (dNTP) concentrations measured

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Creator:: Davis, Ralph Eugene, 1957-
Abstract:: Vaccinia virus infected monkey kidney cells had been previously shown to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA from mutant virus resistant to hydroxyurea were digested with restriction endonucleases and were shown to have substoichiometric amounts of the Hind III F fragment. Additional information from Southern blotting experiments...
Resource Type:: Dissertation
Full Text:: . Intracellularlocalization. and Interaction with a DNA-binding Protein. Abstract approved: Christopher K. Mathews, Ph.D

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Creator:: Spiro, Craig
Abstract:: Vaccinia virus-infected animal cells have been used to study the interactions between the replication of deoxyribonucleic acid (DNA) and the biosynthesis of its nucleotide precursors. Some antimetabolites that inhibit DNA replication have as their targets enzymes of nucleotide biosynthesis. Furthermore, the disruption of nucleotide metabolism can alter the fidelity of...
Resource Type:: Dissertation
Full Text:: Metabolism in Vaccinia Virus-Infected Mammalian Cells. Abstract Redacted for Privacyapproved: Christopher K

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Creator:: Sargent, Roy Geoffrey
Abstract:: Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
Resource Type:: Dissertation
Full Text:: privacy Christopher K. Mathews Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Creator:: Wang, Yeong
Abstract:: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is...
Resource Type:: Dissertation
Full Text:: Acknowledgement I wish to express my sincere thanks to my major advisor, Dr. Christopher K Mathews, for his

Nucleoside diphosphokinase of Escherichia coli and its interactions with bacteriophage T4 proteins of DNA synthesis

Creator:: Ray, Nancy Bisset
Resource Type:: Dissertation
Full Text:: privacy Redacted for privacy Acknowledgements I would like to thank my advisor, Christopher K

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Creator:: Mun, Byung-joo
Abstract:: Although the synthesis of DNA precursors is closely coordinated with DNA replication, it is still not clear whether deoxyribonucleoside triphosphates (dNTPs) influence DNA replication independently of their interactions with DNA polymerase catalytic sites. In an effort to understand the extent to which rate and fidelity of DNA replication are regulated...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Redacted for Privacy Christopher K. Mathews Although the synthesis of DNA precursors

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation
Full Text:: Christopher K. Mathews, Ph.D. The ability to faithfully replicate DNA is dependent upon the maintenance and

ScholarsArchive@OSU

Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

Attempted cloning of T4 phage ribonucleotide reductase

Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

Deoxyribonucleotides as determinants of DNA replication fidelity in bacteriophage T4

Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

Nucleoside diphosphokinase of Escherichia coli and its interactions with bacteriophage T4 proteins of DNA synthesis

Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

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