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Filtering by: Creator Jones, Christopher Remove constraint Creator: Jones, Christopher Subject DNA -- Synthesis Remove constraint Subject: DNA -- Synthesis

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  • Mutagenic mechanisms associated with perturbations of DNA precursor biosynthesis in phage T4

    Creator:
    Ji, Jiuping
    Abstract:
    A crucial factor in determining the accuracy of DNA replication is maintenance of a balanced supply of deoxyribonucleoside triphosphates (dNTPs) at replication forks. Perturbation of dNTP biosynthesis can induce dNTP pool imbalance with deleterious genetic consequences, including increased mutagenesis, recombination, chromosomal abnormalities and cell death. Using the T4 bacteriophage system,...
    Resource Type:
    Dissertation
    Full Text:
    Perturbations of DNA Precursor Biosynthesis in Phage T4 Redacted for Privacy Abstract Approved: Christopher K

  • Studies on DNA precursor metabolism in vaccinia virus-infected mamalian cells

    Creator:
    Spiro, Craig
    Abstract:
    Vaccinia virus-infected animal cells have been used to study the interactions between the replication of deoxyribonucleic acid (DNA) and the biosynthesis of its nucleotide precursors. Some antimetabolites that inhibit DNA replication have as their targets enzymes of nucleotide biosynthesis. Furthermore, the disruption of nucleotide metabolism can alter the fidelity of...
    Resource Type:
    Dissertation
    Full Text:
    Metabolism in Vaccinia Virus-Infected Mammalian Cells. Abstract Redacted for Privacyapproved: Christopher K

  • Attempted cloning of T4 phage ribonucleotide reductase

    Creator:
    Booth, Joseph
    Abstract:
    Ribonucleotide reductase is an important enzyme in the control of DNA replication within the cell. Ribonucleotide reductase exerts its control through enzymatic reduction of nucleoside diphosphates. The bacteriophage T4 enzyme is an example of the class of iron-requiring reductases which also includes E. coli and mammalian ribonucleotide reductases. The two...
    Resource Type:
    Masters Thesis
    Full Text:
    model exist, however (Ator, M. A. and Stubbe, J., 1985; Spector, T. and Jones, T. E., 1985). F. RDPR

  • Modulation of deoxyribonucleoside triphosphate levels, DNA synthesis rates and fidelity in mammalian cells

    Creator:
    Martomo, Stella A.
    Abstract:
    Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
    Resource Type:
    Dissertation
    Full Text:
    . Abstract approved: Christopher K. Mathews Deoxyribonucleoside triphosphate (dNTP) concentrations measured

  • Organization of T4 bacteriophage genes and gene products involved in DNA precursor biosynthesis

    Creator:
    Wang, Yeong
    Abstract:
    Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is...
    Resource Type:
    Dissertation
    Full Text:
    Acknowledgement I wish to express my sincere thanks to my major advisor, Dr. Christopher K Mathews, for his

  • Protein-protein interactions in the bacteriophage T4-coded dCTPase-dUTPase

    Creator:
    Ungermann, Christian
    Resource Type:
    Masters Thesis
    Full Text:
    like to thank Dr. Christopher K. Mathews for the opportunity to work in his laboratory and for his

  • Insights into mammalian DNA replication from analysis of mutations affecting deoxyribonucleotide biosynthesis

    Creator:
    Mun, Byung-joo
    Abstract:
    Although the synthesis of DNA precursors is closely coordinated with DNA replication, it is still not clear whether deoxyribonucleoside triphosphates (dNTPs) influence DNA replication independently of their interactions with DNA polymerase catalytic sites. In an effort to understand the extent to which rate and fidelity of DNA replication are regulated...
    Resource Type:
    Dissertation
    Full Text:
    . Abstract approved: Redacted for Privacy Christopher K. Mathews Although the synthesis of DNA precursors

  • Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

    Creator:
    Davis, Ralph Eugene, 1957-
    Abstract:
    Vaccinia virus infected monkey kidney cells had been previously shown to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA from mutant virus resistant to hydroxyurea were digested with restriction endonucleases and were shown to have substoichiometric amounts of the Hind III F fragment. Additional information from Southern blotting experiments...
    Resource Type:
    Dissertation
    Full Text:
    . Intracellularlocalization. and Interaction with a DNA-binding Protein. Abstract approved: Christopher K. Mathews, Ph.D

  • Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

    Creator:
    Young, James Patrick
    Abstract:
    The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
    Resource Type:
    Dissertation
    Full Text:
    Christopher K. Mathews, Ph.D. The ability to faithfully replicate DNA is dependent upon the maintenance and

  • Sequence conversion during adenovirus DNA replication

    Creator:
    Bennett, Kelly L.
    Abstract:
    The work in this thesis has provided conclusive genetic evidence that "panhandle" intermediates form during adenovirus replication. Adenovirus chromosomes lacking 51 by from their left -hand termini are infectious and capable of regenerating the missing origin sequence. Yet if an entire inverted terminal repeat is removed, the adenovirus chromosome is...
    Resource Type:
    Dissertation
    Full Text:
    extend my gratitude to my committee members, Dr. Christopher Mathews, Dr. Pui Shing Ho, Dr. Dal lice