Index Catalog // ScholarsArchive@OSU

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Creator:: Thompson, Cynthia Sue
Abstract:: The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Resource Type:: Dissertation
Full Text:: long, depending on the species. In E. coli, S8 is 129 amino acids long with a molecular weight of

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Creator:: Kaller, Margo
Abstract:: Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Resource Type:: Masters Thesis
Full Text:: Figure 4. Molecular structure of locked nucleic acid (LNA) antisense oligomers. The “Base” in both

The discriminator domain : does it reside at the C-terminus or the N-terminus of Escherichia coli Lon?

Creator:: Miller, Darcey L.
Abstract:: The mechanisms of substrate recognition by regulatory proteases are not well understood. Presently, two opposing models have arisen to describe E. coil Lon's ability to discriminate between substrates: one suggests the N-terminus involvement while the second suggests the C-terminus involvement. In this project, the role of the C-terminal domain as...
Resource Type:: Masters Thesis
Full Text:: Reagents and Enzymes 30 Bacterial Strains, Plasmids and Media 30 Nucleic Acid Extraction 33 Small Scale

The function and structural characteristics of conserved regions within Escherichia Coli small subunit ribosomal RNA

Creator:: Almehdi, Mirza A.
Abstract:: Ribosomes are multicomponent macromolecular particles and are essential for the survival of cells in all organisms. The ribosome's universal function is to catalyze polypeptide synthesis through translation of mRNA transcripts. Ribosomes from Escherichia coli, eubacterial organisms, have a sedimentation coefficient of 70S and are composed of 30S and 50S ribonucleoprotein...
Resource Type:: Dissertation
Full Text:: ., Dondon, J., Grunberg-Manago, M., Ebel, J.P. and Ehresmann, B. (1986) Nucleic Acid Res. 14, 4803- 4821

Accessibility of highly conserved RNA sequences of Escherichia coli 70S ribosomes and implications for developing antibacterial agents

Creator:: Rahman, Mohammad A.
Abstract:: Escherichia coli 16S-like ribosomal RNAs from eubacterial, archaebacterial and eukaryotic organisms have several universally conserved sequence zones which are proposed to be single-stranded. A clear function for all of these regions has not yet been defined. To evaluate the roles of these sequence zones in protein synthesis, knowledge of their...
Resource Type:: Dissertation
Full Text:: formation of polypeptide chains from activated amino acid residues. The polymerization of amino acids into

Generation and in vitro assembly evaluation of a site-specific deletion in Escherichia coli small subunit ribosomal RNA

Creator:: Yoo, Young Sook
Abstract:: Ribosomes are intricate macromolecular complexes which are a major element of the protein biosynthetic machinery in all life forms. In Escherichia coli they contain about 50 distinct proteins and 3 ribosomal RNAs. The small 30S ribosomal subunit in E. coli incorporates 21 proteins and a 16S rRNA. The 16S rRNA...
Resource Type:: Dissertation
Full Text:: Boom, J. H., Veenemann, G., and van Duin, J. (1981) Nucleic Acids Res. 9, 1425-1444. 26. Suryanarayana

The effect of streptomycin on proline synthesis in Escherichia coli

Creator:: Couch, David Bruce
Abstract:: The effect of streptomycin on an early reaction of proline synthesis, the production of glutamic-γ-semialdehyde, in resting cell suspensions of antibiotic-sensitive, resistant, and dependent strains of Escherichia coli was examined, and the effect on glutamic-γ-semialdehyde production was found to correspond to the growth response of the organisms to streptomycin. Inhibition...
Resource Type:: Masters Thesis
Full Text:: Concentration 42 The Effect of Nucleic Acid 42 The Mode of Action of Adenine and Nucleic Acid 45 The Effect

The association of DNA with other cellular materials in Escherichia coli lysates

Creator:: Porter, Bruce Wallace
Abstract:: The association of other materials with DNA has been studied extensively in higher organisms and more recently in bacteria. However, investigation of these complexes in bacteria have for the most part been restricted to the study of a specific function this association may serve, or a specific kind of association....
Resource Type:: Dissertation
Full Text:: phosphate groups of nucleic acid. How- ever, since basic amino acids comprised only 20 percent of the

Inactivation of bacterial pathogens in drinking water

Creator:: Hartlerode, Evelyn R.
Abstract:: Wine is known to have been enjoyed since ancient times. Although early civilizations where unaware of scientific mechanisms of the properties of wine, empirical methods allowed them knowledge of its intoxicating effects as well as its healthful properties. These properties lead the ancients to use wine for many purposes including...
Resource Type:: Masters Thesis
Full Text:: ). Unsaturated fatty acids inhibited cellular fatty acid synthesis (Zheng and others 2005). Unsaturated fatty

Site-specific 3'-terminal deletions in Escherichia coli 16S rRNA and the effect on assembly of 30S ribosomal subunits

Creator:: Abdul-Latif, Fazilah
Abstract:: The ribosome is a central component of the protein synthetic apparatus. It is a macromolecular complex of protein and RNA. Although much progress has been made in understanding the functional role of the proteins in this particle, little is known of the functional role which the RNA plays. Naturally occurring...
Resource Type:: Masters Thesis
Full Text:: 23S Ribosomal RNA. Nucleic Acids Res. 9: 6167-6189. 15. Dahlberg, A.E., Lund, E., Kjeldgaard, N.O

ScholarsArchive@OSU

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

The discriminator domain : does it reside at the C-terminus or the N-terminus of Escherichia coli Lon?

The function and structural characteristics of conserved regions within Escherichia Coli small subunit ribosomal RNA

Accessibility of highly conserved RNA sequences of Escherichia coli 70S ribosomes and implications for developing antibacterial agents

Generation and in vitro assembly evaluation of a site-specific deletion in Escherichia coli small subunit ribosomal RNA

The effect of streptomycin on proline synthesis in Escherichia coli

The association of DNA with other cellular materials in Escherichia coli lysates

Inactivation of bacterial pathogens in drinking water

Site-specific 3'-terminal deletions in Escherichia coli 16S rRNA and the effect on assembly of 30S ribosomal subunits

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