Index Catalog // ScholarsArchive@OSU

The discriminator domain : does it reside at the C-terminus or the N-terminus of Escherichia coli Lon?

Creator:: Miller, Darcey L.
Abstract:: The mechanisms of substrate recognition by regulatory proteases are not well understood. Presently, two opposing models have arisen to describe E. coil Lon's ability to discriminate between substrates: one suggests the N-terminus involvement while the second suggests the C-terminus involvement. In this project, the role of the C-terminal domain as...
Resource Type:: Masters Thesis
Full Text:: Reside at the C-terminus or the N-terminus of Escherichia coli Lon? Abstract approved: Janine E. Trempy

Survival Assessment, Inactivation Kinetics Models, and Farm-to-Table Quantitative Risk Analysis of Food Pathogens

Creator:: Deng, Kai
Abstract:: About 48 million cases of Foodborne illnesses (FBIs) are estimated to occur every year in the US. These are diseases caused by contamination of foods with bacteria, viruses, protozoa, parasites and toxic chemicals. Clostridium difficile infections (CDIs) are an important health-care and community associated problem. Since C. difficile spores have...
Resource Type:: Dissertation
Full Text:: community associated problem. Since C. difficile spores have been frequently isolates from meat product

Generation and in vitro assembly evaluation of a site-specific deletion in Escherichia coli small subunit ribosomal RNA

Creator:: Yoo, Young Sook
Abstract:: Ribosomes are intricate macromolecular complexes which are a major element of the protein biosynthetic machinery in all life forms. In Escherichia coli they contain about 50 distinct proteins and 3 ribosomal RNAs. The small 30S ribosomal subunit in E. coli incorporates 21 proteins and a 16S rRNA. The 16S rRNA...
Resource Type:: Dissertation
Full Text:: ., and Woese, C. R. (1986) Cell 45, 325-326. 46. Prince, J. B., Taylor, B. H., Thurlow, D. L., Ofengand

Recovery of Escherichia coli from freeze dried model systems

Creator:: Hirway, Sumesh Chandra
Resource Type:: Masters Thesis
Full Text:: media studied were Violet Red Bile agar, Desoxycholate agar, Eosin Methylene Blue agar, E., C

Removal of E. coli with alternative media Biosand filters

Creator:: Fulton, Nathan J.
Abstract:: When Biosand filters cannot be constructed with crushed quarry rock due to resource limitations, a suitable alternative filter media is needed. In this research, two crushed quarry rock alternatives were examined. Three bench-scale Biosand filters with crushed rock, beach sand, and heat-treated beach sand media were simultaneously dosed with Willamette...
Resource Type:: Masters Thesis
Full Text:: . Taylor Water Treatment Plant and for allowing me to use their facilities. Lucy Gibson of the Oregon

Coliphage T3 S-adenosylmethionine hydrolase : properties of the cloned gene and consequences of its expression in Escherichia coli

Creator:: Hughes, Jeffrey A.
Abstract:: To begin the development of a research tool for use in the in vivo study of S-adenosylmethionine (SAM) metabolism, I attempted to locate, isolate, clone and elicit the expression of the SAM hydrolase gene (E.C. 3.3.1.2) of the coliphage T3. The gene was isolated and cloned into the M13mp8 cloning...
Resource Type:: Dissertation
Full Text:: donated by other researchers. I wish to thank Drs. C. Mathews, D. Morris, M. Marinus, G. Walker, and B

The function and structural characteristics of conserved regions within Escherichia Coli small subunit ribosomal RNA

Creator:: Almehdi, Mirza A.
Abstract:: Ribosomes are multicomponent macromolecular particles and are essential for the survival of cells in all organisms. The ribosome's universal function is to catalyze polypeptide synthesis through translation of mRNA transcripts. Ribosomes from Escherichia coli, eubacterial organisms, have a sedimentation coefficient of 70S and are composed of 30S and 50S ribonucleoprotein...
Resource Type:: Dissertation
Full Text:: . The proper structure is shown in Figure 1-5. c°0 X000000 30S Subunit 16S rRNA rProteins (si ,s2

Accessibility of highly conserved RNA sequences of Escherichia coli 70S ribosomes and implications for developing antibacterial agents

Creator:: Rahman, Mohammad A.
Abstract:: Escherichia coli 16S-like ribosomal RNAs from eubacterial, archaebacterial and eukaryotic organisms have several universally conserved sequence zones which are proposed to be single-stranded. A clear function for all of these regions has not yet been defined. To evaluate the roles of these sequence zones in protein synthesis, knowledge of their...
Resource Type:: Dissertation
Full Text:: plot for DNA 520 65 c) Saturation curve of DNA 1396 66 d) Scatchard plot for DNA 1396 67 Figure 3-4

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Creator:: Thompson, Cynthia Sue
Abstract:: The effects of oligoribonucleotides of identical or similar sequences to a ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Resource Type:: Dissertation
Full Text:: intermediate) that is the rate limiting step to 30S particles. When the reaction is carried out at 30°C and

Mass spectrometric studies on peptides and proteins : conformations of Escherichia coli Thioredoxin and its alkylated adducts studied by hydrogen/deuterium exchange and HPLC-electrospray ionization mass spectrometry

Creator:: Kim, Moo-young
Abstract:: E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...
Resource Type:: Dissertation

ScholarsArchive@OSU

The discriminator domain : does it reside at the C-terminus or the N-terminus of Escherichia coli Lon?

Survival Assessment, Inactivation Kinetics Models, and Farm-to-Table Quantitative Risk Analysis of Food Pathogens

Generation and in vitro assembly evaluation of a site-specific deletion in Escherichia coli small subunit ribosomal RNA

Recovery of Escherichia coli from freeze dried model systems

Removal of E. coli with alternative media Biosand filters

Coliphage T3 S-adenosylmethionine hydrolase : properties of the cloned gene and consequences of its expression in Escherichia coli

The function and structural characteristics of conserved regions within Escherichia Coli small subunit ribosomal RNA

Accessibility of highly conserved RNA sequences of Escherichia coli 70S ribosomes and implications for developing antibacterial agents

Oligoribonucleotides directed at ribosomal protein binding sites as inhibitors of ribosomal small subunit assembly and as possible antibiotics

Mass spectrometric studies on peptides and proteins : conformations of Escherichia coli Thioredoxin and its alkylated adducts studied by hydrogen/deuterium exchange and HPLC-electrospray ionization mass spectrometry

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