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Filtering by: Alternative title ssim EC 1076 Subject Escherichia coli -- Genetics

Characterization of Escherichia coli double-strand uracil-DNA glycosylase and analysis of uracil-initiated base excision DNA repair

Creator:

Sung, Jung-Suk

Abstract:

Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized with respect to activity, substrate specificity, product DNA binding, and mechanism of action. Purified Dug excised both uracil...

Resource Type:

Dissertation

Full Text:

....................................................................................................... 233 5.1.1 Comparison of Substrate Specificity of Dug on U/G and eC/G DNA
Escherichia coli uracil-DNA glycosylase : DNA binding, catalysis, and mechanism of action

Creator:

Shroyer, Mary Jane N.

Resource Type:

Masters Thesis

Full Text:

to recognize 3,N4-ethenocytosine (EC) paired to guanine (46, 115) and thymine paired to O6- methyl
Mechanism of action of Escherichia coli uracil-DNA glycosylase and interaction with the bacteriophage PBS-2 uracil-DNA glycosylase inhibitor protein

Creator:

Lundquist, Amy J.

Resource Type:

Dissertation
Characterization of the Escherichia coli uracil-DNA glycosylase- inhibitor protein interaction

Creator:

Bennett, Samuel E.

Resource Type:

Dissertation

Full Text:

E. coli deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) plays a central role