Chlamydiae are obligate intracellular bacteria that infect a variety of
eukaryotic hosts and affect normal host processes. Within host cells, their
developmental cycle takes place inside non-acidified vacuoles termed inclusions. An
inclusion membrane composed primarily of secreted chlamydial synthesized proteins
called Incs encloses the inclusion. At this location, Incs have...
The dataset here is used as a document file for presenting supplementary videos in the dissertation Tao, K. (2018). Investigating protein-lipid-membrane interactions in Plant Cells using Bimolecular Fluorescence Complementation (Doctoral dissertation).
Keywords: Protein, Lipid, Membrane, Plant Cells, Fluorescent Imaging
The Chlamydiaceae are a family of obligate intracellular bacteria with a unique biphasic developmental cycle. The replicating, metabolically active, intracellular phase bacteria (RBs) grow within a cellular vacuole know as the inclusion. Since the proteins the Chlamydiaceae secrete into this membrane are the means of host/parasite interaction, they have long...
The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...
Expression of membrane-type matrix metalloproteinases (MT) and tissue inhibitors of matrix metalloproteinases (TIMP) was evaluated in sheep embryos and uterus during the pre- and peri-implantation periods. Embryos and uterine samples were surgically collected from ewes on days 9, 11, 13, and 15 of pregnancy (n=3 ewes/day) and of the estrous...
The pathogen Vibrio cholerae uses cations as a primary currency of virulence
and environmental persistence, using gradients of those cations to move, acquire
nutrients, and control virulence gene expression. An understanding of the overlapping
roles of bioenergetics and chemotaxis in the virulence and environmental survival of
V. cholerae issues from...
Studies were conducted to determine the effects of cyclopropenoid
fatty acids (CPFA) on the microsomal membrane of livers of rainbow
trout (Salmo gairdneri). Slab and tube gel electrophoresis of
microsomes from trout fed a CPFA diet (CPFA-microsomes) for varying
time periods showed a decrease in the number of protein bands...
Pseudomonas is a diverse genus of Gram-negative bacteria that includes pathogens of plants, insects, and humans as well as environmental strains with no known pathogenicity. Pseudomonas fluorescens itself encompasses a heterologous group of bacteria that are prevalent in soil and on foliar and root surfaces of plants. Some strains of...
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Structural analysis of the pyoverdine TonB-dependent outer-membrane protein .81
Iron-limited