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Filtering by: Subject Plant gene expression

Agrobacterium-mediated transformation of hybrid poplar (Populus alba x P. grandidentata) and analysis of foreign gene expression

Creator:

Howe, Glenn Thomas

Abstract:

A method for Agrobacterium-mediated transformation of hybrid poplar (Populus alba x P. grandidentata) suspension cultures and regeneration of transformed plants is described. The best protocol was one in which suspension cultures were inoculated with Agrobacterium tumefaciens to a density of 10⁷ cfu's/ml, cocultivated for 48 hours, plated to cellulose acetate...

Resource Type:

Dissertation
Growth, morphology, and in vitro development of transgenic poplars with RNAi-inhibited PtDDM1-1/2 gene expression

Creator:

Zhu, Ruoqing

Abstract:

Epigenetic mechanisms are important for control of plant development, and may play a particularly important role in trees given their long life cycles and distinctive and stable tissue types. To help understand the role of epigenetics in tree development, we produced transgenic poplars with reduced activity of the DDM1 genes,...

Resource Type:

Masters Thesis
Functions and regulation of cytokinin glucosyltransferases

Creator:

Pineda Rodó, Albert

Abstract:

Cytokinins can be conjugated to N- and O-glucosides. While N-glucosylation generally leads to irretrievable loss of activity, O-glucosides are thought to play an important role in cytokinin storage and homeostasis since they can be hydrolyzed to the corresponding aglycones. The first zeatin O-glycosyltransferase genes isolated were ZOG1 and ZOX1 from...

Resource Type:

Dissertation