Preliminary studies were run to determine the sensitivity and
reliability of enzyme-linked immunosorbent assay (ELISA) for the
detection of potato virus X (PVX). ELISA was found to be capable of
detecting PVX in tobacco at a 1:3333 sample dilution. The reliability
of ELISA for the detection of PVX in potato...
The reliability and sensitivity of enzyme-linked immunosorbent
assay (ELISA) in the detection of ilarvirus (isometric labile ringspot
virus) subgroup B and prune dwarf virus (PDV) isolates in Prunus and
Malus species has been well established. To use this technique in
large scale virus indexing programs more information was required on...
The lentil strain of pea seedborne mosaic virus, PSbMV-L, was
distinguished from the standard isolates of PSbMV by a natural inoculum
reservoir in lentil seed and by separate resistance genes in Pisum
and Lens. PSbMV-L was intermediate in symptom severity between the
standard and New Zealand isolates of PSbMV.
Seed...
The location of prune dwarf virus (PDV) and Prunus
necrotic ringspot virus (PNRSV) in pollen and seed
from infected and healthy sweet cherry trees pollinated
with infected pollen was investigated. Virus-like
particles were observed in the cytoplasm of pollen
grains from PDV-infected cherry trees using transmission
electron microscopy. No particles...
Pea seed-borne mosaic potyvirus pathotype 1 (PSbMV-P1) has the ability to infect most genotypes of Pisum sativum. The exception are those genotypes that are homozygous recessive for the sbm-1 gene. The life cycle of PSbMV pathotype 4 (PSbMV-P4) is unaffected by the sbm-1/sbm-1 genotype. Infectious clones of Pl-P4 recombinants were...
Raspberry bushy dwarf virus (RBDV) is the sole member of the idaeovirus genus of plant viruses. It is a pollen-borne virus that economically impacts both red and black raspberries worldwide. Three strains of the bipartite RBDV have been reported. The common strain found in North America, South America, Australia, New...
Pallidosis is a disease of strawberry identified in 1957 with an unknown etiology. Two previously uncharacterized viruses in strawberry, Strawberry pallidosis associated virus (SPaV) and Beet pseudo-yellows virus (BPYV), have been found associated with disease symptoms. The complete nucleotide sequence of both viruses was determined and molecular detection protocols developed....