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Filtering by: Creator Sukhatme, Gaurav S. Subject Rainbow trout -- Molecular genetics

Characterization of Pacific whiting proteinase P-II and partial cloning of cathepsins L and K cDNA from rainbow trout liver

Creator:

Nickel, Xianbin F.

Abstract:

Proteinase P-II purified from parasitized Pacific whiting muscle was previously identified to be one form of cathepsin L. It appeared to be present in three isozymatic forms on non-denaturing PAGE gel stained for activity. Its autolytic degradation was observed on SDS-PAGE gel under its optimum condition, 55°C and pH 5.5,...

Resource Type:

Masters Thesis

Full Text:

Properties of Cathepsins B, H, L, and S 4 Tissue Distribution of Cathepsins B, L, and S 6 Sequence
Molecular characterization of the MX genes of rainbow trout (Oncorhynchus mykiss)

Creator:

Trobridge, Grant David

Abstract:

The Mx protein family of rainbow trout (Oncorhynchus mykiss) was characterized at the molecular level. With primers derived from a partial sequence of Perch (Perca fluviatis) Mx genomic DNA, a partial rainbow trout Mx genomic DNA sequence was obtained by polymerase chain reaction (PCR) amplification. All salmonid fish investigated contained...

Resource Type:

Dissertation

Full Text:

from Perch (Perca f7uviatis) (Staeheli et al., 1989) led us to attempt cloning the Mx protein(s) of