A method for rapid isolation of plasmid DNA from group N
streptococci was developed. Chief advantages of the method were
simplicity, the utilization of microliter quantities of reagents,
and the obtainment of preparations highly enriched for covalently
closed circular plasmid DNA. The method was also applied to over
15 Leuconostoc...
The public health and economic significance of rapid acid production
by lactic streptococci in controlled dairy fermentations is well
known. However, the fast acid-producing characteristic of these organisms
is not stable, and cultures of fast organisms have been shown
to contain slow cells. These studies were carried out to characterize...
Streptococcus gordonii (S. gordonii) is being developed as a Gram-positive
expression vector for the expression of heterologous proteins. This bacterial
expression system takes advantage of the natural pathway of protein export and
anchoring on the surface of the bacterium. The expression of heterologous proteins
relies on the homologous recombination of...
The dairy industry relies primarily on consistent acid production
by the lactic streptococci for the manufacture of certain cheeses
and fermented dairy products. Variation in these cultures due to
genetic exchange has not been thoroughly investigated. This study
was undertaken to determine if genetic homology exists within the lactic
group,...
Comparisons were made of the abilities of Streptococcus lactis,
Streptococcus cremoris and Streptococcus diacetilactis bacteriophages
to endure various laboratory isolation and storage treatments.
Neutralization of Cottage cheese whey containing phages offered no
survival advantage over unneutralized samples, refrigeration of
neutral and acid whey samples increased the survival of only the...
The tendency for certain single- and mixed-strain lactic
starter cultures to develop a fruity and/or fermented off-flavor
in ripening Cheddar cheese was traced to certain strains of
Streptococcus lactis in the starter. A very intense fruity and/or
fermented flavor developed in experimental cheeses when one particular
S. lactis strain was...