Pactamycin, a potent antitumor antibiotic produced by the soil bacterium Streptomycespactum, is a structurally unique aminocyclopentitol-containing natural product. Itconsists of a highly functionalized cyclopentitol core unit, two aromatic rings [3-aminoacetophenone (3AAP) and 6-methylsalicylic acid (6MSA)], and a 1,1-dimethylurea moiety. Despite its potent biological activity, the development of thisantibiotic was hampered...
The discovery of new antibiotics reached its peak during the golden era of antibiotics in the mid 20th century and continued to thrive until the 1980s. However, after more than 50 years of fruitful exploration and exploitation of microbial natural products the discovery rate of new antibiotics has dropped drastically...
This investigation was undertaken to provide a chemical
description of an actinophage for Streptomyces griseus, and to
determine the base composition of the phage DNA moiety. It was
believed of interest to learn whether the actinophage for the
filamentous S. griseus host would be different in its chemical
properties from...
Cysteine inhibition of phage multiplication in Escherichia
coli has been described by a number of workers. More recently
cysteine has been found to lower the yield of Streptomyces griseus
phage. The present study sought a more complete description of the
"cysteine effect" as it has been noted in the S....
The problem investigated here is part of a larger project designed
to study the nature and function of the fine structure of Streptomyces
griseus phage 514-3. The aims of this portion of the project
are to attempt to disrupt intact S. griseus phage by methods that reportedly
disrupted T even...
Glucose metabolism of phage-infected Streptomyces griseus
was examined by using the specific radioactivities of the amino
acids from the extracted protein of the host and phage as an index
of the catabolic pathways of glucose metabolism. Conventional
methods for the study of phage-infected bacteria did not suit the
host-actinophage system...
A modified agar overlay technique for the preliminary screening
of antibiotic producing Streptomyces spp. has been developed and
compared to the routinely used shake flask method and the
unmodified agar overlay technique. The percentage of isolates
exhibiting antimicrobial activities was found to be greater in the
modified overlay technique.
Three...