Vaccinia virus (VV) is a large DNA virus belonging to the Orthopoxvirus
family. The viral replicative life cycle takes place solely within the cytoplasm
of a mammalian host cell. The VV genome contains 196 open reading frames
which are expressed in a highly regulated and temporal fashion in order to...
Vaccinia virus-infected animal cells have been used to
study the interactions between the replication of
deoxyribonucleic acid (DNA) and the biosynthesis of its
nucleotide precursors. Some antimetabolites that inhibit
DNA replication have as their targets enzymes of nucleotide
biosynthesis. Furthermore, the disruption of nucleotide
metabolism can alter the fidelity of...
The attachment of myristic acid to the N-terminal glycine residue of many
eukaryotic and viral proteins is often essential for the acquisition of the protein's
biological activity. Vaccinia virus (VV), the prototype member of the Poxviridae,
expresses several myristylated proteins during the course of infection. Only one of
these proteins,...
Vaccinia virus encodes at least eight proteins that are modified post-translationally by the addition of a 16-carbon saturated fatty acid through thioester linkage to cysteine residues. This is referred to as palmitylation of proteins. The purpose of this work was to gain an understanding of palmitylation, focusing on what defined...
Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting
step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project
was to characterize the ribonucleotide reductase encoded by the orthopoxvirus,
vaccinia. The first objective was to study the structural and functional features of the
viral small subunit...
Vaccinia virus (VV) encodes at least six proteins that are modified by the addition of a 14-carbon saturated fatty acid through an amide linkage and at least eight proteins that are modified post-translationally by the addition of a 16-carbon saturated fatty acid through linkage to cysteine residues. These post-translational modifications...