Sulfite mutants representing five complementation groups, previously derived from
an ethyl methanesulfonate-treated haploid strain of Saccharomyces cerevisiae were studied.
Although the wildtype S. cerevisiae strain used (isogenic to X2180-1 A) had a basal
tolerance for sulfite (7 μM free H₂SO₃), the sensitive and resistant mutants were found to
tolerate less...
RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs, including transacting siRNAs (tasiRNAs), arise from transcripts targeted by some microRNAs (miRNAs). In the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)-miR390 complexes interact with primary transcripts at two sites, resulting in recruitment...
Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
The yeast Brettanomyces bruxellensis is the most important wines spoilage yeast encountered during winemaking as it can survive in wine for long periods, requires minimal nutrients for growth, and can be difficult to control. Brettanomyces produces two major spoilage products, the volatile phenols 4-ethylphenol (Band-Aid, medicinal smell) and 4-ethylguaiacol (smoke,...
The composition and distribution of fish assemblages was examined in a
floodplain lake system in the Amazon basin. Quantitative samples were
collected during the 1992-1993 flooding season at Marchantaria Island, Solimoes
River. A total of 25,819 specimens representing 8 orders, 30 families, 101
genera and 139 species of fish were...
In plantation forests, competition from unwanted vegetation may reduce survival and negatively impact tree growth. The goal of this study was to examine the influence of vegetation management treatments on plant water relations and wood properties. Control trees (no treatment) were compared to trees subjected to post-planting competing vegetation control...
Red radish anthocyanin extract (RAE) was investigated for coloring brined
cherries as an alternative to FD&C Red No. 40. Red radish (Raphanus sativus L.)
anthocyanins were extracted from liquid nitrogen powdered epidermal tissue using
acetone, partitioned with chloroform, and isolated using C-18 resin. The monomeric
anthocyanin content was determined by...
Red radish (Raphanus sativus L.) anthocyanins were extracted from liquid
nitrogen powdered epidermal tissue using acetone and chloroform and isolated using
C-18 resin. Chemical structure and conformation of major pigments were elucidated
by 1-D and 2-D NMR, Mass Spectroscopy, HPLC, and spectral analyses. Two novel
di-acylated anthocyanins, pelargonidin 3-0-[2-0-(β-D-glucopyranosyl)-(6-0- trans-p-coumaroyl-...
Chipping varieties and model systems were used to determine the role of potato
constituents on chip color. Composition was evaluated by HPLC and chip color
measured using a ColorQuest colorimeter. Reducing sugar (RS) content did not
completely explain color quality when present in low concentrations (<60 mg/lOOg).
Levels of ascorbic...
Background: Against global health disparities and a shortfall of medical personnel, community health workers (CHWs) promise a solution based in lived experience and connection to the health system. To address global and upstream health determinants, CHW programs engage the perspectives of both the workers they employ and the communities they...
This thesis uses both feminist and new historic theories to argue that the women's romance novels The Last September (1929) by Elizabeth Bowen and The Country Girl's Trilogy (1960, 1962, 1964) by Edna O'Brien are tragic bildungsroman that subvert and challenge the Irish patriarchal marriage expectations of their respective time...