PCR-based codon-specific random mutagenesis and site-specific mutagenesis were performed to construct a library of 18 amino acid changes at Arg276 in the conserved leucine-loop of the core catalytic domain of human uracil-DNA glycosylase (UNG). Each Arg276 mutant was then overproduced in E. coli cells and purified to apparent homogeneity by...
What constitutes quality of learning in primary care? A
social systems view of that central question regards the
relationships between dimensions of learning, purposes of
primary care, and quality of practice. The question of
learning quality was approached in three ways. First,
perceptions of learning quality were elicited through
recorded...