The development of micro total analysis systems (µTAS), also called “lab-on-a-chip”, or microfluidic analysis systems, is presented in this dissertation. Various research areas, covering subjects from magnetic particles synthesis to novel microchip fabrication techniques, are explored to develop a lab-on-a-chip system capable of performing magnetic bead-based bioassays. These devices are...
The objective of this thesis is to introduce and demonstrate a novel magnetic bead detector based on inductive detection at the ferromagnetic resonance (FMR) frequency for use in bio-sensing applications. Detection ability is demonstrated through theoretical arguments, numerical computer simulations, and experimental characterization of micro-fabricated detectors.
The detector is composed...
Detection technique that proposed utilizing electrochemically controlled, reversible ion extraction into polymeric membrane in an alternating galvanostatic/ potentiostatic mode was introduced. This method is studied in detail to comprehend the advantages of the novel sensor (pulstrode) compared to the potentiometric ion selective electrode (ISE), studies included possible applications and limitations....
Approximately 12% of Americans do not consume the amount of zinc equal to the Estimated Average Requirement for zinc and could be at risk for marginal zinc deficiency. Zinc is an essential component of proteins in the defense against oxidative stress and DNA damage repair. Specifically in the prostate, zinc...
The emerging field of nanotechnology strictly requires the micro-scaling of the available separation technology and the design of novel devices for separations of molecules of interest. The separation of proteins and nanoparticles is challenging due to their relatively large size, non-specific adherence to surfaces and instability in many solvents.
This...
Dielectrophoresis (DEP) is a separation method in which a non-uniform electric
field is used to induce a dipole moment in a suspended particle. If the polarization
of the particle is greater than that of the suspending medium, the particle will move
towards the region of higher field strength (positive DEP);...
While replacement of dietary saturated fat with unsaturated fat has been advocated
to reduce cardiovascular disease risk, diets high in polyunsaturated fatty acids
(PUFA) could increase low density lipoprotein (LDL) susceptibility to oxidation,
potentially contributing to the pathology of atherosclerosis. To assess in vivo lipid
peroxidation and susceptibility, of LDL...
This report details preliminary studies towards the development of a microfluidic
sensor that exploits ferromagnetic resonance, excited in magnetic bead labels, for signal
transduction. The device consists of a microwave circuit in which a slotline and a coplanar
waveguide are integrated with a biochemically activated sensor area. The magnetic beads...
Full Text:
2011
Chatterjee, Esha
Department of Chemistry, chattere@onid.orst.edu, Oregon State
This report details preliminary studies towards the development of a microfluidic
sensor that exploits ferromagnetic resonance, excited in magnetic bead labels, for signal
transduction. The device consists of a microwave circuit in which a slotline and a coplanar
waveguide are integrated with a biochemically activated sensor area. The magnetic beads...
Full Text:
Microsoft Word - Biographies[1].doc
Biographies:
Esha
This report details preliminary studies towards the development of a microfluidic
sensor that exploits ferromagnetic resonance, excited in magnetic bead labels, for signal
transduction. The device consists of a microwave circuit in which a slotline and a coplanar
waveguide are integrated with a biochemically activated sensor area. The magnetic beads...