The effects of different nitrogen sources on the primary and secondary pathways of Azotobacter vinelandii cells were studied by means of the radiorespirometric method. C¹⁴ labeled glucose and several key amino acids related to the tricarboxylic acid cycle, such as glutamic acid, aspartic acid, alanine, serine and glycine were used...
Nutritional studies with Acetobacter suboxydans indicated that
when isoleucine, lysine, methionine, serine and arginine were singly
omitted from a complete amino acid mixture, no growth was obtained.
However, the organism could grow on single amino acids like glutamic
acid, histidine and proline. No growth was obtained with ammonium
sulfate as...
Comparatively little work has been done on bacterial lipids.
There are profound differences between bacterial lipids in general
and lipids of the higher forms of life in such respects as the absence
of sterols, phospholipids low in nitrogen but high in inositol
and carbohydrate, the presence of large proportions of...
This investigation was undertaken to develop a procedure
by which the fatty acids could be separated from the
hydroxy fatty acids of bark waxes. The specific hexane-insoluble, benzene-soluble wax used in this research was obtained
from the bark of white fir, Abias concolor, (Lindl.
and Gord.).
The acids were removed...
In this study subcutaneous, inter- and intramuscular fats from 42
Hereford steers were analyzed for their fatty acid composition using
gas-liquid chromatography. The same tissues were analyzed from one
pair each of identical twins of Angus and Shorthorn breeding. Muscle
and liver lipids from these latter four animals were fractionated...
Routine studies of bacterial fatty acid and lipid composition,
made possible by recently developed techniques, have brought about
an increasing interest in the physiological significance of these
compounds. The purpose of this study was to characterize the fatty
acids of the vegetative cells of Clostridium botulinum 33A, as the
first...
The difficulties encountered in working with micro-quantities
of lipids have been overcome by the advent of new and improved
procedures in chromatography which have made possible better
fractionation and identification. Bacterial lipids because of their
different lipid pattern from other life forms have stimulated much
interest. Studies have been made...
Female Sprague-Dawley rats (220-240 g) were ovariectomized
and seven days later were either treated as controls or received a
single injection of either 0.1 μg, 100 μg, or 2 mg of estradiol-17β dissolved in 0.1 cc propylene glycol. These rats were autopsied
either 1, 3, 6, 24, 48, or 72...