Partial purification of diamine oxidase from pea seedlings,
Pisum sativum, was accomplished by homogenization of 8-10 day
etiolated epicotyl tissue, followed by ammonium sulfate fractionation
and DEAE Sephadex column chromatography. The preparation thus
obtained was purified 50 fold.
Some properties of this enzyme were investigated. A radio-tracer method was adapted...
Three types of experiments were designed to further characterize
the tryptophan decarboxylating enzyme of Reed and Crecelius
(1967).
1) Light inhibition studies were made with etiolated pea seedlings
to determine the in vitro response of the tryptophan decarboxylating
enzyme. Assays of indoleacetic acid oxidase (IAA oxidase)
and guaiacol-active pea seedling...
Several aspects of the potent inhibition of bovine plasma amine
oxidase (PAO) by hydrazines were investigated by kinetic and preparative
means. The inhibition was classified as pseudo-irreversible
by the method of Ackerman and Potter (Proc. Soc. Exptl. Biol. Med.
72, 1 (1949), and was found to exhibit Zone B kinetic...
The presence in rat liver microsomes of an enzyme system
which demethylates N-methylhydrazines and azo compounds to formaldehyde
has been reported. Some of the compounds found to be
substrates were monomethylhydrazine, N-isopropyl-α-(2-methylhydrazino)-
p-toluamide and the azo derivative of the latter hydrazine. This
enzyme can be classified as a mixed function...
An aspect of the metabolism of monoalkylhydrazines has been
investigated using both intact rats and rat liver microsomal enzyme
preparations.
Intact rats respired propane and n-butane on administration of 0.3-0.5 mmoles of isopropyl- and n-butylhydrazine, respectively.
Based upon dose administered, a five to ten percent
yield of hydrocarbon was obtained....
The biochemical degradation of domestic sludge deposits exposed to benthic marine conditions was investigated using a laboratory Deep Sea Simulator. The rate and extent of deposit stabilization were determined for select hydrostatic pressures and hydrogen acceptor conditions by monitoring depletion of particulate organic carbon in 2.0 and 3.0 centimeter deposits....
The ability of N,N'-diphenylurea (DPU) to substitute for cytokininactive
adenine derivatives in promoting callus growth of Phaseolus lunatus
and Nicotiana tabacum has been examined. In general, DPU stimulated
callus growth of P. lunatus at high concentrations and the growth of
most callus tissues was irregular, while the response of tobacco...