Different strains of Vibrio parahaemolyticus (Vp) in broth cultures and Vp-inoculated live Pacific oysters (Crassostrea gigas) were subjected to high pressure processing (HPP) at 241, 276, 310, and 345 MPa. Results showed Vp numbers were reduced by HPP in both pure culture and whole oysters. Vp inactivation was dependent on...
Microorganisms play key roles in ocean biogeochemistry. However, several
predominant groups of uncultured bacterioplankton thought to contribute to
important biogeochemical processes in the oceans are known primarily from gene
cloning studies. Although these studies have greatly expanded our view of
microbial diversity in the oceans, they are not quantitative and...
A new high-throughput culturing (HTC) method using a low nutrient
heterotrophic medium (LNHM) has led to the isolation of many novel strains of
oligotrophic bacteria from marine ecosystems. Four strains belonging to a single
dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated
from the coast of Oregon by the HTC...
The phylogenetic diversity of two continental shelf picoplankton
communities was examined by analyzing SSU (16S) ribosomal RNA (rRNA)
genes amplified from environmental DNA with bacterial-specific primers and
the polymerase chain reaction (PCR). Picoplankton populations collected from
the pycnocline (10 m) over the eastern continental shelf of the United States
near...
Symbiotic relationships of bacteria with higher organisms are
commonly observed in nature; however, the functional role of these
relationships is only rarely understood. This is particularly evident in
epibiotic bacterial associations in the marine environment where the bacteria
are often a diverse ensemble of microorganisms, thus complicating the
identification of...
The objective of this project was to use ribosomal RNA genes, cloned
from natural populations of Sargasso Sea bacterioplankton, as markers for
picoplankton diversity. It is widely recognised that a majority of
microorganisms have yet to be cultivated, and therefore much of extant
microbial diversity remains unknown (50). The method...
Biological di-nitrogen (N₂) fixation is a key process in open-ocean ecosystems, where the new nitrogen (N) provided by marine diazotrophs can support a large fraction of primary productivity and carbon (C) drawdown. Recent laboratory studies have shown that elevated pCO₂ enhances the rate of N₂ fixation by select laboratory isolates...
Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...